T cell recognition of cell‐surface antigens. II. Antigen recognition is necessary yet not sufficient for triggering T cell‐mediated immunity
T cell recognition of cell‐surface antigens. II. Antigen recognition is necessary yet not sufficient for triggering T cell‐mediated immunity
复制标题
T 细胞对细胞表面抗原的识别是必要的，但不足以触发 T 细胞介导的免疫。
DOI：
10.1002/eji.1830040404
复制
发表时间：
1974
影响因子：
5.4
通讯作者：
M. Feldman
中科院分区：
文献类型：
--
作者：
H. Wekerle;E. Kölsch;M. Feldman
我们研究了淋巴细胞对细胞表面抗原本身的识别是否是触发T细胞致敏的必要和充分条件。在活的或戊二醛固定的成纤维细胞上培养5天后，我们通过测量对与致敏单层相同的靶单层的细胞毒性来检测正常大鼠淋巴细胞对小鼠成纤维细胞单层的致敏性。活的或戊二醛处理的成纤维细胞单层被用作细胞免疫吸附剂来检测识别。
We investigated whether recognition per se of cell surface antigens by lymphocytes is both necessary and sufficient to trigger sensitization of T cells. We assayed sensitization of normal rat lymphocytes against monolayers of mouse fibroblasts by measuring the acquisition of cytotoxicity against target monolayers syngeneic to the sensitizing monolayer, following 5 days of culturing on living or glutaraldehyde‐fixed fibroblasts. Monolayers of either living or glutaraldehyde‐treated fibroblasts were used as cellular immunoadsorbents to assay recognition.