G-quadruplexes in telomeric repeats are conserved in a solvent-free environment
G-quadruplexes in telomeric repeats are conserved in a solvent-free environment
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DOI：
10.1016/j.ijms.2006.03.016
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发表时间：
2006-07-01
影响因子：
1.8
通讯作者：
Bowers, Michael T.
中科院分区：
文献类型：
--
作者：
Baker, Erin Shammel;Bernstein, Summer L.;Bowers, Michael T.
g -四相形成层序的构造性质。例如人类端粒重复序列d(T(2)AG(3))(n)，由于它们在癌症和细胞衰老中的作用而引起了人们的极大兴趣。确定在无溶剂环境中是否存在g -四聚物。利用质谱、离子迁移率和分子动力学计算研究了不同长度的端粒重复序列d(T(2)AG(3)) n （n = 1、2、4和6）和dTG(4)T。纳米esi - ms表征与g -四联结构相容的四联化学计量对于每个序列，dT(2)AG(3)和dTG(4)T与两个和三个NH4+加合物形成4链配合物。d(T(2)AG(3))(2)一个2链配合物。d(T(2)AG(3))(4)和d(T(2)AG(3))(6)为单链。用离子迁移率法得到了所有物种的实验截面。无论如何……这些可以定量匹配具有特定链取向（平行/反平行）和结构的模型横截面。对于每个物种，无溶剂结构与溶液CD测量值一致，但离子迁移率/建模过程通常提供更详细的结构信息。(C) 2006 Elsevier B.V.版权所有
The structural properties of G-quadruplex forming sequences. such as the human telomeric repeat d(T(2)AG(3))(n), are of great interest due to their role in cancer and cellular aging. To determine if G-quadruplexes are present in a solvent-free environment. different lengths of the telomeric repeat d(T(2)AG(3))(n) (where n = 1, 2, 4 and 6) and dTG(4)T were investigated with mass specteometry, ion mobility and molecular dynamics calculations. Nano-ESI-MS illustrated quadruples stoichiometries compatible with G-quadruplex Structure.; for each sequence, with dT(2)AG(3) and dTG(4)T forming 4-strand complexes with two and three NH4+ adducts. d(T(2)AG(3))(2) a 2-strand complex. and d(T(2)AG(3))(4) and d(T(2)AG(3))(6) remaining single-stranded. Experimental cross sections were obtained for all species using ion mobility method.,. In all case.,. these could be quantitatively matched to model cross sections with specific strand orientations (parallel/antiparallel) and structures. For each species, the solvent-free structures agreed with the solution CD measurements, but the ion mobility/modeling procedure often gave Much more detailed structural information. (C) 2006 Elsevier B.V. All rights reserved.