Methods for studying toxicity of silica-based nanomaterials to living cells.
Methods for studying toxicity of silica-based nanomaterials to living cells.
复制标题
研究二氧化硅基纳米材料对活细胞毒性的方法。
DOI：
10.1007/978-1-62703-468-5_15
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Zhao,JuliaXiaojun
中科院分区：
文献类型：
--
作者：
Zhao,Yang;Jin,Yuhui;Hanson,Aaron;Wu,Min;Zhao,JuliaXiaojun
近年来，已经开发了许多基于二氧化硅的纳米材料。这些纳米材料的一个重要应用是在生物和生物医学应用领域。然而，已经提出了关于纳米材料在体外的安全性的主要担忧。为了解决这个问题，已经开发了几种方法来系统地研究基于二氧化硅的纳米颗粒的细胞毒性和遗传毒性。这些方法主要是在传统毒性研究方法的基础上进行了一些改进。本章总结了硅基纳米材料毒性研究的四种重要方法。这些方法可以检测细胞增殖、细胞活力、DNA损伤和活性氧（ROS）的产生。详细介绍了每种方法的协议。
A number of silica-based nanomaterials have been developed in recent years. An important application of these nanomaterials is in the field of biological and biomedical applications. However, a major concern about the safety of the nanomaterials in vitro has been proposed. To address this problem, several approaches have been developed for a systematic investigation of the cytotoxicity and genotoxicity of silica-based nanoparticles. These methods are mainly based on the traditional toxicity study approaches but with some modifications. In this chapter, four important methods for studying of toxicity of silica-based nanomaterials are summarized. These methods can detect cell proliferation, cell viability, DNA damage, and the generation of reactive oxygen species (ROS). The protocols of each method are introduced in detail.