Overexpression of Shadoo protein in transgenic mice does not impact the pathogenesis of scrapie
Overexpression of Shadoo protein in transgenic mice does not impact the pathogenesis of scrapie
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DOI：
10.1016/j.neulet.2011.03.073
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发表时间：
2011-05
影响因子：
2.5
通讯作者：
Haiying Wang;Jiayu Wan;Wei Wang;Dongxu Wang;Sha Li;Peng Liao;Zhuo Hao;Songbo Wu;Jing Xu;Nan Li;Hongsheng Ouyang;Hongwei Gao
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文献类型：
--
作者：
Haiying Wang;Jiayu Wan;Wei Wang;Dongxu Wang;Sha Li;Peng Liao;Zhuo Hao;Songbo Wu;Jing Xu;Nan Li;Hongsheng Ouyang;Hongwei Gao
Shadoo是一种在成人大脑中表达的糖蛋白，是朊病毒蛋白的相互作用蛋白;然而，其功能仍有待确定。为了阐明其在朊病毒发病机制中的作用，我们产生了由鼠PrP启动子驱动的过表达野生型（wt）Shadoo的转基因小鼠。murineSprn转基因的表达显著增加了产生的所有三种小鼠系中的脑Shadoo蛋白水平。在感染小鼠适应性羊瘙痒病菌株22 L后，所有测试的转基因小鼠都表现出羊瘙痒病的特征。重要的是，Shadoo的表达水平或孵育时间与疾病表型之间没有相关性。因此，我们得出结论，Shadoo对转基因小鼠的传染性海绵状脑病（TSE）疾病的结果几乎没有影响。
Shadoo is a glycoprotein expressed in the adult brain that is an interacting protein of prion protein; however, its function remains to be determined. To elucidate its role in prion pathogenesis, we generated transgenic mice overexpressing wild-type (wt) Shadoo driven by the murine PrP promoter. Expression of the murineSprntransgene significantly increased brain Shadoo protein levels in all three mouse lines generated. Following infection with mouse-adapted scrapie strain22L, all transgenic mice tested exhibited characteristics of scrapie disease. Importantly, there was no correlation between the expression level or incubation time of Shadoo with disease phenotype. We therefore conclude that Shadoo has little or no influence on the outcome of transmissible spongiform encephalopathy (TSE) disease in transgenic mice.