PFOS, PFNA, and PFOA sub-lethal exposure to embryonic zebrafish have different toxicity profiles in terms of morphometrics, behavior and gene expression.
PFOS, PFNA, and PFOA sub-lethal exposure to embryonic zebrafish have different toxicity profiles in terms of morphometrics, behavior and gene expression.
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DOI：
10.1016/j.aquatox.2016.03.026
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Cooper KR
中科院分区：
文献类型：
--
作者：
Jantzen CE;Annunziato KA;Bugel SM;Cooper KR
多氟化合物（PFC）是一类人为的、持久性的有毒化学品。全氟化碳在世界范围内都被检测到，由不同长度、末端基团和工业用途的氟化碳链组成。文献中先前的斑马鱼研究以及我们自己的研究表明，暴露于低浓度范围（0.02 µM - 2.0 µM; 20-2000 ppb）的这些化学物质会导致化学特定的发育缺陷和孵化后存活率降低。据推测，亚致死胚胎暴露于全氟辛烷磺酸（PFOS），全氟壬酸（PFNA），或全氟辛酸（PFOA）将导致不同的反应方面的形态，行为和基因表达在卵黄囊鱼苗和斑马鱼幼虫。斑马鱼在受精后的前五天（dpf）暴露于PFOS、PFOA和PFNA（0.02、0.2、2.0 µM），并分析了形态计量学（5 dpf、14 dpf）、靶基因表达（5 dpf、14 dpf）和运动行为（14 dpf）。所有三种PFC通常导致总体长减少，在5dpf时增加tfc 3a（肌肉发育）表达和减少ap 1 s（蛋白质转运）表达，以及在14 dpf时过度活跃的运动活动。在两个生命阶段时间点测量的所有其他终点在每种PFC之间变化。全氟辛烷磺酸、全氟萘甲酸和全氟辛酸暴露导致在形态测量、运动和基因表达终点方面的反应发生显著改变，这可以在实地暴露的硬骨鱼中表现出来。
Polyfluorinated compounds (PFC) are a class of anthropogenic, persistent and toxic chemicals. PFCs are detected worldwide and consist of fluorinated carbon chains of varying length, terminal groups, and industrial uses. Previous zebrafish studies in the literature as well as our own studies have shown that exposure to these chemicals at a low range of concentrations (0.02 µM – 2.0 µM; 20–2000 ppb) resulted in chemical specific developmental defects and reduced post hatch survival. It was hypothesized that sub-lethal embryonic exposure to perfluorooctanesulfonic acid (PFOS), perfluorononanoic acid (PFNA), or perfluorooctanoic acid (PFOA) would result in different responses with regard to morphometric, behavior, and gene expression in both yolk sac fry and larval zebrafish. Zebrafish were exposed to PFOS, PFOA, and PFNA (0.02, 0.2, 2.0 µM) for the first five days post fertilization (dpf) and analyzed for morphometrics (5 dpf, 14 dpf), targeted gene expression (5 dpf, 14 dpf), and locomotive behavior (14 dpf). All three PFCs commonly resulted in a decrease in total body length, increased tfc3a (muscle development) expression and decreased ap1s (protein transport) expression at 5dpf, and hyperactive locomotor activity 14 dpf. All other endpoints measured at both life-stage time points varied between each of the PFCs. PFOS, PFNA, and PFOA exposure resulted in significantly altered responses in terms of morphometric, locomotion, and gene expression endpoints, which could be manifested in field exposed teleosts.
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