Natural Variations in SLG7 Regulate Grain Shape in Rice
Natural Variations in SLG7 Regulate Grain Shape in Rice
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SLG7 的自然变异调节水稻的粒形
DOI：
10.1534/genetics.115.181115
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发表时间：
2015-12-01
期刊：
影响因子：
3.3
通讯作者：
Liang, Guohua
中科院分区：
文献类型：
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作者：
Zhou, Yong;Miao, Jun;Liang, Guohua
水稻（Oryza sativa）粒形由数量性状基因座（QTL）控制，对产量和品质有很大影响。然而，谷物发育的分子基础仍然很大程度上未知。在这项研究中，我们利用源自 9311 和 Azucena 的回交渐渗系，鉴定了一个新的 QTL，即 7 号染色体上的细长粒 (SLG7)，它负责粒状。 Azucena 的 SLG7 等位基因产生更长、更薄的籽粒，但它对籽粒重量和产量没有影响。 SLG7 编码与 LONGIFOLIA 1 和 LONGIFOLIA 2 同源的蛋白质，这两种蛋白都会增加拟南芥中的器官长度。 SLG7在水稻各组织中组成型表达，SLG7蛋白定位于质膜。形态学和细胞分析表明，SLG7 通过纵向增加细胞长度，同时横向减少细胞宽度来产生细长的颗粒，这与细胞分裂无关。我们的研究结果表明，SLG7 家族成员的功能在单子叶植物和双子叶植物中都是保守的，并且 SLG7 等位基因可以应用于育种以改变水稻籽粒外观。
Rice (Oryza sativa) grain shape, which is controlled by quantitative trait loci (QTL), has a strong effect on yield production and quality. However, the molecular basis for grain development remains largely unknown. In this study, we identified a novel QTL, Slender grain on chromosome 7 (SLG7), that is responsible for grain shape, using backcross introgression lines derived from 9311 and Azucena. The SLG7 allele from Azucena produces longer and thinner grains, although it has no influence on grain weight and yield production. SLG7 encodes a protein homologous to LONGIFOLIA 1 and LONGIFOLIA 2, both of which increase organ length in Arabidopsis. SLG7 is constitutively expressed in various tissues in rice, and the SLG7 protein is located in plasma membrane. Morphological and cellular analyses suggested that SLG7 produces slender grains by longitudinally increasing cell length, while transversely decreasing cell width, which is independent from cell division. Our findings show that the functions of SLG7 family members are conserved across monocots and dicots and that the SLG7 allele could be applied in breeding to modify rice grain appearance.