An element upstream from the human delta-globin-encoding gene specifically enhances beta-globin reporter gene expression in murine erythroleukemia cells.
An element upstream from the human delta-globin-encoding gene specifically enhances beta-globin reporter gene expression in murine erythroleukemia cells.
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人δ珠蛋白编码基因上游的元件特异性增强鼠红白血病细胞中β珠蛋白报告基因的表达。
DOI：
10.1016/0378-1119(96)83098-8
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发表时间：
1996
期刊：
影响因子：
3.5
通讯作者：
Bank,A
中科院分区：
文献类型：
--
作者：
Acuto,S;Urzi,G;Schimmenti,S;Maggio,A;O'Neill,D;Bank,A
We have previously shown that a DNA-binding factor specific to adult hematopoietic cells (polypyrimidine-binding factor; PYBF) binds to a pyrimidine-rich region 1 kb upstream from the human δ-globin-encoding gene (HBD) The developmental stage-specificity of PYBF and the location of its binding site between the fetal and adult β-globin (HBB)-like genes suggest that PYBF and its binding site may function in fetal-to-adult globin gene switching. Here, we describe the effect of 383-bp (δ383) and 99-bp (δ99) sequences containing the PYBF-binding site on transcription from various globin and non-globin promoters, using a transient assay with the cat reporter gene in murine erythroleukemia (MEL) cells, a cell line with abundant PYBF activity. We show that both δ383 and δ99 specifically enhance expression of cat for plasmids containing a human adult globin (HBB) promoter, whereas expression of similar constructs using human fetal (Aγ-) globin (HBG1) or simian virus 40 (SV40) promoters is not enhanced. The results suggest that PYBF and the pyrimidine-rich region upstream from HBD can specifically enhance HBB transcription in adult erythroid cells.
