Early diagnosis of lymph node metastasis: Importance of intranodal pressures.
Early diagnosis of lymph node metastasis: Importance of intranodal pressures.
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DOI：
10.1111/cas.12873
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发表时间：
2016-03
期刊：
影响因子：
5.7
通讯作者：
Kodama T
中科院分区：
文献类型：
--
作者：
Miura Y;Mikada M;Ouchi T;Horie S;Takeda K;Yamaki T;Sakamoto M;Mori S;Kodama T
区域淋巴结状态是肿瘤侵袭性的重要预后指标。然而，在淋巴结大小没有显着变化的阶段，利用结内压力对转移进行早期诊断尚未进行研究。在这里，我们使用MXH10/Mo-lpr/lpr小鼠淋巴结转移模型来表明，当肿瘤细胞注射到髂下淋巴结以诱导转移至固有腋窝淋巴结时，通过淋巴管连接的髂下淋巴结和固有腋窝淋巴结的结内压力都会增加。我们发现，在通过体内生物发光检测到转移的阶段，髂下淋巴结的结内压力增加，但当适当的腋窝淋巴结体积（通过高频超声成像测量）没有显着增加。通过体内生物发光在实体瘤中检测到封装吲哚菁绿的静脉注射脂质体，但在适当的腋窝淋巴结中未检测到。尽管检测到窦组织细胞增多症，但正确的腋窝淋巴结仍保留基本的血管和淋巴管结构。这些结果表明，当转移性肿瘤细胞尚未完全增殖时，固有腋窝淋巴结的结内压力在早期阶段就会增加。结内压可能是促进淋巴结转移早期诊断的有用参数。
Regional lymph node status is an important prognostic indicator of tumor aggressiveness. However, early diagnosis of metastasis using intranodal pressure, at a stage when lymph node size has not changed significantly, has not been investigated. Here, we use an MXH10/Mo‐lpr/lpr mouse model of lymph node metastasis to show that intranodal pressure increases in both the subiliac lymph node and proper axillary lymph node, which are connected by lymphatic vessels, when tumor cells are injected into the subiliac lymph node to induce metastasis to the proper axillary lymph node. We found that intranodal pressure in the subiliac lymph node increased at the stage when metastasis was detected by in vivo bioluminescence, but when proper axillary lymph node volume (measured by high‐frequency ultrasound imaging) had not increased significantly. Intravenously injected liposomes, encapsulating indocyanine green, were detected in solid tumors by in vivo bioluminescence, but not in the proper axillary lymph node. Basic blood vessel and lymphatic channel structures were maintained in the proper axillary lymph node, although sinus histiocytosis was detected. These results show that intranodal pressure in the proper axillary lymph node increases at early stages when metastatic tumor cells have not fully proliferated. Intranodal pressure may be a useful parameter for facilitating early diagnosis of lymph node metastasis.