Differential immune responses of Monochamus alternatus against symbiotic and entomopathogenic fungi
Differential immune responses of Monochamus alternatus against symbiotic and entomopathogenic fungi
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松墨天牛对共生真菌和昆虫病原真菌的差异免疫反应
DOI：
10.1007/s11427-017-9102-y
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Sun J.H.
中科院分区：
文献类型：
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作者：
Zhang W.;Meng J.;Ning J.;Qin P.J.;Zhou J.;Zou Z.;Wang Y.H.;Jiang H.;Ahmad F.;Zhao L.L.;Sun J.H.
松墨天牛（Monochamusalternatus）是入侵松材线虫的主要媒介昆虫，与中国本土的一种外营养共生真菌孢子丝菌（Sporothrixsp.1）建立了共生关系。免疫反应的M.松墨天牛S. sp.1在甲虫和真菌共存中的作用尚不清楚。在这里，我们报告的免疫反应M。松墨天牛对外营养共生真菌S. sp.1与白僵菌（Beauveria bassiana）的差异显著。色葡萄sp.1不能杀死金龟子，而B.巴西纳注射后全部死亡。转录组结果显示，在M.松墨天牛感染S. sp.1的感染率比感染B的感染率低2倍。在感染后48小时，人们注意到，当被共生真菌感染时，Toll和IMD通路在甲虫的免疫系统中起主导作用，但当被昆虫病原真菌感染时，只有Toll通路被主动触发。此外，通过在48 h追溯Toll和IMD途径，甲虫可以耐受共生真菌的感染。本研究为M.为进一步研究宿主和相关真菌之间的免疫相互作用提供了参考。
Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response of M. alternatus to S. sp. 1 in the coexistence of beetles and fungi is, however, unknown. Here, we report that immune responses of M. alternatus pupae to infection caused by ectotrophic symbiotic fungus S. sp. 1 and entomopathogenic fungus Beauveria bassiana differ significantly. The S. sp. 1 did not kill the beetles while B. bassiana killed all upon injection. The transcriptome results showed that the numbers of differentially expressed genes in M. alternatus infected with S. sp. 1 were 2-fold less than those infected with B. bassiana at 48 hours post infection. It was noticed that Toll and IMD pathways played a leading role in the beetle’s immune system when infected by symbiotic fungus, but upon infection by entomopathogenic fungus, only the Toll pathway gets triggered actively. Furthermore, the beetles could tolerate the infection of symbiotic fungi by retracing their Toll and IMD pathways at 48 h. This study provided a comprehensive sequence resource of M. alternatus transcriptome for further study of the immune interactions between host and associated fungi.