Characterization of amphioxus AmphiWnt8:: insights into the evolution of patterning of the embryonic dorsoventral axis
Characterization of amphioxus AmphiWnt8:: insights into the evolution of patterning of the embryonic dorsoventral axis
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DOI：
10.1046/j.1525-142x.2000.00047.x
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发表时间：
2000-03-01
影响因子：
2.9
通讯作者：
Holland, ND
中科院分区：
文献类型：
--
作者：
Schubert, M;Holland, LZ;Holland, ND
本文报道了文昌鱼Wnt基因AmphiWnt 8的全长序列及其发育表达。在文昌鱼胚胎中，AmphiWnt 8的表达模式表明在前脑、后肠和近轴中胚层中发挥模式化作用，从而产生肌肉体节。系统发育分析表明，一个单一的Wnt 8亚家族基因在祖先脊索动物复制到Wnt 8进化枝和Wnt 8b进化枝的脊椎动物进化早期。与这种基因复制相一致，祖先AmphiWnt 8样基因的功能似乎已经在脊椎动物Wnt 8b（专门神经源性的，特别是在前脑中）和脊椎动物Wnt 8（杂项，特别是在早期体节发生中）之间划分。文昌鱼AmphiWnt 8及其脊椎动物Wnt 8同源物可能在胚胎体轴的早期背腹图案中发挥类似的作用。
The full-length sequence and developmental expression of an amphioxus Wnt gene (AmphiWnt8 ) are described. In amphioxus embryos, the expression patterns of AmphiWnt8 suggest patterning roles in the forebrain, in the hindgut, and in the paraxial mesoderm that gives rise to the muscular somites. Phylogenetic analysis indicates that a single Wnt8 subfamily gene in an ancestral chordate duplicated early in vertebrate evolution into a Wnt8 clade and a Wnt8b clade. Coincident with this gene duplication, the functions of the ancestral AmphiWnt8-like gene appear to have been divided between vertebrate Wnt8b (exclusively neurogenic, especially in the forebrain) and vertebrate Wnt8 (miscellaneous, especially in early somitogenesis). Amphioxus AmphiWnt8 and its vertebrate Wnt8 homologs probably play comparable roles in the early dorsoventral patterning of the embryonic body axis.