Dissecting the regulatory circuitry of a eukaryotic genome
Dissecting the regulatory circuitry of a eukaryotic genome
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DOI：
10.1016/s0092-8674(00)81641-4
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发表时间：
1998-11-25
期刊：
影响因子：
64.5
通讯作者：
Young, RA
中科院分区：
文献类型：
--
作者：
Holstege, FCP;Jennings, EG;Young, RA
全基因组表达分析用于鉴定其表达取决于酵母中转录起始机制的关键组分的功能的基因。RNA聚合酶II全酶，一般的转录因子TFIID，和佐贺染色质修饰复合物的组件被发现有不同的基因组的表达中的作用。结果揭示了一个意想不到的调节水平，这是叠加在由于基因特异性转录因子，一种新的机制，协调调节特定的基因组，当细胞遇到限制营养物质，并证明信号转导途径的最终目标可以确定内的起始装置。
Genome-wide expression analysis was used to identify genes whose expression depends on the functions of key components of the transcription initiation machinery in yeast. Components of the RNA polymerase II holoenzyme, the general transcription factor TFIID, and the SAGA chromatin modification complex were found to have roles in expression of distinct sets of genes. The results reveal an unanticipated level of regulation which is superimposed on that due to gene-specific transcription factors, a novel mechanism for coordinate regulation of specific sets of genes when cells encounter limiting nutrients, and evidence that the ultimate targets of signal transduction pathways can be identified within the initiation apparatus.