Wnt11r Is Required for Cranial Neural Crest Migration
Wnt11r Is Required for Cranial Neural Crest Migration
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DOI：
10.1002/dvdy.21758
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发表时间：
2008-11-01
影响因子：
2.5
通讯作者：
Mayor, Roberto
中科院分区：
文献类型：
--
作者：
Matthews, Helen K.;Broders-Bondon, Florence;Mayor, Roberto
wnt11r是最近发现的Wnt基因家族的成员，它被认为是非洲爪蟾与哺乳动物wnt11基因的真正同源物。在这项研究中，我们研究了wnt11r在神经嵴发育中的作用。对wnt11r的表达分析以及与神经嵴标志物snail2和非典型Wnt wnt11的比较表明，wnt11r表达于神经嵴内侧或神经板侧，而wnt11表达于外侧或表皮侧。注射wnt11r morpholino对神经嵴迁移有较强的抑制作用，对神经嵴诱导和维持无影响。这种效果可以通过联合注射Wnt11r而不是wnt11mrna来恢复，这表明了功能丧失治疗的特异性。最后，神经嵴移植实验表明，wnt11r以非细胞自主的方式控制神经嵴迁移是必需的。科学通报，2009(7):344 - 344。(C) 2008 Wiley-Liss, Inc。
wnt11r is a recently identified member of the Wnt family of genes, which has been proposed to be the true Xenopus homologue to the mammalian wnt11 gene. In this study we have examined the role of wnt11r on neural crest development. Expression analysis of wnt11r and comparison with the neural crest marker snail2 and the noncanonical Wnt, wnt11, shows wnt11r is expressed at the medial or neural plate side of the neural crest while wnt11 is expressed at the lateral or epidermal side. Injection of wnt11r morpholino leads to strong inhibition of neural crest migration with no effect on neural crest induction or maintenance. This effect can be rescued by co-injection of Wnt11r but not by Wnt11 mRNA, demonstrating the specificity of the loss of function treatment. Finally, neural crest graft experiments show that wnt11r is required in a non-cell-autonomous manner to control neural crest migration. Developmental Dynamics 237:3404-3409, 2008. (C) 2008 Wiley-Liss, Inc.