Single molecule thermodynamics in biological motors
Single molecule thermodynamics in biological motors
复制标题
DOI：
10.1016/j.biosystems.2006.08.016
复制
发表时间：
2007-04-01
期刊：
影响因子：
1.6
通讯作者：
Yanagida, Toshio
中科院分区：
文献类型：
--
作者：
Taniguchi, Yuichi;Karagiannis, Peter;Yanagida, Toshio
生物分子机器利用热活化能来执行各种功能。热激活过程具有输出事件的随机性质，可以根据热力学定律进行描述。最近开发的单分子检测技术允许对单个生物机器的每个不同酶促事件进行表征，从而为潜在的热力学提供线索。在这项研究中，研究了生物分子马达步进运动的热力学特性。使用单分子检测技术测量不同负载和温度下的步进运动，并获得与每个前进和后退的产生相关的一系列热力学参数，包括自由能、焓、熵和特征距离。结果表明，熵的不对称性是控制电机步进方向的主要因素。单分子热力学的研究有可能揭示生物分子机器工作机制背后的动态特性。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
Biological molecular machines use thermal activation energy to carry out various functions. The process of thermal activation has the stochastic nature of output events that can be described according to the laws of thermodynamics. Recently developed single molecule detection techniques have allowed each distinct enzymatic event of single biological machines to be characterized providing clues to the underlying thermodynamics. In this study, the thermodynamic properties in the stepping movement of a biological molecular motor have been examined. A single molecule detection technique was used to measure the stepping movements at various loads and temperatures and a range of thermodynamic parameters associated with the production of each forward and backward step including free energy, enthalpy, entropy and characteristic distance were obtained. The results show that an asymmetry in entropy is a primary factor that controls the direction in which the motor will step. The investigation on single molecule thermodynamics has the potential to reveal dynamic properties underlying the mechanisms of how biological molecular machines work. (c) 2006 Elsevier Ireland Ltd. All rights reserved.