Seizure susceptibility in mice: Differences in brain ATP productionin vitro
Seizure susceptibility in mice: Differences in brain ATP productionin vitro
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小鼠癫痫发作易感性：体外脑 ATP 产生的差异
作者：
J. MacInnes;W. Boggan;K. Schlesinger
研究了稀释和非稀释品系小鼠的粗品和纯化的脑和肝线粒体制剂中ADP向ATP的转化。ATP生产被认为是明显更大的脑线粒体从14日龄，21日龄和35日龄的稀释小鼠;最大的差异，观察到在21天。在肝线粒体中ADP向ATP的转化率无显著性差异，在脑线粒体中的差异似乎不是由于稀释小鼠中ATP酶活性降低所致。发现稀释的21日龄DBA/2 J小鼠的全脑匀浆中的ATP比21日龄未稀释的C57 BL/6 J小鼠多约25%。这些发现表明，在稀基因型小鼠中氧化磷酸化和癫痫易感性之间存在相关性。由于在DBA/2 J和C57 BL/6 J菌株之间，以及在稀释致死（d1/d1）和同窝非致死对照（D/d1）之间发现了ATP产生的类似差异，因此该位点似乎与此有关。
Conversion of ADP to ATP in crude and purified brain and liver mitochondrial preparations from dilute and non-dilute strains of mice was studied. ATP production was found to be appreciably greater in brain mitochondria from 14-, 21-, and 35-day old dilute mice; maximum differences were observed at 21 days. No significant differences in conversion of ADP to ATP in liver mitochondrial preparations were found. The differences in brain mitochondria from these mice seemed not to be due to decreased ATPase activity in dilute mice. Dilute 21-day old DBA/2J mice were found to have approximately 25% more ATP in whole brain homogenates than 21-day old non-dilute C57BL/6J mice. These findings suggest a correlation between oxidative phosphorylation and seizure susceptibility in mice of dilute genotypes. Since similar differences in ATP production were found between the DBA/2J and C57BL/6J strains, and between dilute lethal (d1/d1) and littermate non-lethal controls (D/d1), thed locus would seem to be implicated.