The influence of cations on aerobic sporogenesis in a liquid medium
The influence of cations on aerobic sporogenesis in a liquid medium
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DOI：
10.1128/jb.26.6.543-558.1933
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发表时间：
1933-12-01
影响因子：
3.2
通讯作者：
Bryan, CS
中科院分区：
文献类型：
--
作者：
Fabian, FW;Bryan, CS
一价阳离子的氯盐Na、K、NH4和Li（也称为乳酸钠）的影响；二价阳离子Mg、Mn、Ba、Co、Pb和Ni；三价阳离子Al、Ce、Fe；研究了四价阳离子Sn在液体培养基中对孢子发生的影响。采用枯草芽孢杆菌、蜡样芽孢杆菌、肠系膜芽孢杆菌和大芽孢杆菌4种需氧孢子形成菌。将一定摩尔浓度的上述盐置于1.0%蛋白胨溶液中，与微生物接种，18小时后。孵育后，用Anjeszky孢子染色法测定孢子形成。所选的3种摩尔浓度分别是(a)最大摩尔浓度。刺激，(b)在max。由于盐的毒性作用，生存力和不生长，以及(c)质量浓度低于最大值。低盐浓度引起的刺激不足导致的生存能力。测定了pH对孢子发生的影响。结果表明，一价盐的阳离子促进了孢子的发生；双价、三价和四价盐的阳离子没有影响。孢子形成在最大值时最为丰富。刺激。在有利的生长范围内，培养基的pH对孢子的形成没有实质性的影响。最大的摩尔浓度。所研究的4种生物受到的刺激与其他研究人员先前确定的大肠杆菌的刺激相一致。因此，这项工作证实并扩展了我们对阳离子对细菌生存能力的生理作用的认识。
The in-fluence of the chloride salts of the univalent cations, Na, K, NH4 and Li (also Na lactate); the bivalent cations, Mg, Mn, Ba, Co, Pb and Ni; the trivalent cations Al, Ce, and Fe; and of the quadrivalent cation Sn on sporogenesis in a liquid medium was studied. 4 aerobic spore-formers, Bacillus subtilis, B. cereus, B. mesentericus and B. megatherium, were used. Definite molal concentrations of the above salts were placed in 1.0% peptone solution, inoculated with the organisms, and after 18 hrs. incubation, spore formation was determined by Anjeszky''s spore stain. The 3 molalities selected were (a) the one giving max. stimulation, (b) a molality at a point between max. viability and no growth due to toxic effect of salt, and (c) a molality at a point lower than that of max. viability due to insufficient stimulation caused by low salt concentration. The influence of pH on sporogenesis was also determined. Results showed that cations of univalent salts stimulated sporogenesis; cations of bi-, tri[long dash], and quadrivalent salts exerted no influence. Spore formation was most abundant at the point of max. stimulation. Within a favorable growing range, pH of medium did not materially affect spore formation. The molal concentrations at which max. stimulation occurred for the 4 organisms studied corresponded with that for Escherichia coli as previously determined by other investigators. This work, therefore, confirms and extends our knowledge of the physiological effect of cations on bacterial viability.