WORTMANNIN INHIBITS INSULIN-STIMULATED BUT NOT CONTRACTION-STIMULATED GLUCOSE-TRANSPORT ACTIVITY IN SKELETAL-MUSCLE
WORTMANNIN INHIBITS INSULIN-STIMULATED BUT NOT CONTRACTION-STIMULATED GLUCOSE-TRANSPORT ACTIVITY IN SKELETAL-MUSCLE
复制标题
DOI：
10.1016/0014-5793(95)00147-2
复制
发表时间：
1995-03-13
期刊：
影响因子：
3.5
通讯作者：
HOLLOSZY, JO
中科院分区：
文献类型：
--
作者：
LEE, AD;HANSEN, PA;HOLLOSZY, JO
在骨骼肌中，葡萄糖转运受到胰岛素、收缩和缺氧的刺激。在这项研究中，我们使用磷脂酰肌醇3-激酶（PI 3-kinase）抑制剂渥曼青霉素（wortmannin）来检测（i）PI 3-kinase活性是否是胰岛素刺激骨骼肌中葡萄糖转运所必需的，以及（ii）PI 3-kinase是否介导收缩/缺氧刺激葡萄糖转运的途径中的一个步骤。渥曼青霉素完全阻断胰岛素和胰岛素样生长因子-1刺激的肌肉葡萄糖转运。与此相反，渥曼青霉素没有效果的刺激葡萄糖转运的收缩或缺氧，提供证据表明PI 3-激酶活性不参与这些刺激的葡萄糖转运的激活。
In skeletal muscle, glucose transport is stimulated by insulin, contractions and hypoxia, In this study, we used the phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor wortmannin to examine whether (i) PI 3-kinase activity is necessary for stimulation of glucose transport by insulin in muscle, and (ii) PI 3-kinase mediates a step in the pathway by which contractions/ hypoxia stimulate glucose transport. Wortmannin completely blocked insulin- and insulin-like growth factor-1-stimulated glucose transport in muscle. In contrast, wortmannin had no effect on the stimulation of glucose transport by contractions or hypoxia, providing evidence that PI 3-kinase activity is not involved in the activation of glucose transport by these stimuli.