Molecular cloning, expression profiles and characterization of a glutathione reductase in Hevea brasiliensis
Molecular cloning, expression profiles and characterization of a glutathione reductase in Hevea brasiliensis
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巴西橡胶树谷胱甘肽还原酶的分子克隆、表达谱和表征
DOI：
10.1016/j.plaphy.2015.07.022
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发表时间：
2015-11-01
影响因子：
6.5
通讯作者：
Li, Dejun
中科院分区：
文献类型：
--
作者：
Deng, Zhi;Zhao, Manman;Li, Dejun
谷胱甘肽还原酶（GR; EC 1.8.1.7）是一种重要的氧化还原酶，可以保护生物体免受各种氧化应激。本研究从巴西橡胶树（Hevea brasiliensis）中分离到了一个新的GR基因HbGR2。HbGR2 cDNA全长1674 bp，编码557个氨基酸，与其他植物的叶绿体GRs具有较高的同源性。HbGR2定位于烟草叶肉原生质体叶绿体中。在HbGR2的启动子区域预测了与应激或激素反应相关的顺式调控元件。实时RT-PCR分析结果显示，HbGR2在叶片的不同组织和发育阶段均有表达。HbGR2除与攻穗板干燥（TPD）有关外，还受乙烯利（ET）、茉莉酸甲酯（MeJA）、干旱、低温、高盐、伤害和过氧化氢（H2O2）等处理的调控。过表达HbGR2的大肠杆菌（e.c oli）细胞在高浓度H2O2下的耐受性和存活率显著提高，提示HbGR2可能在橡胶树氧化应激反应中发挥重要作用。(C) 2015 Elsevier Masson SAS。版权所有。
Glutathione reductase (GR; EC 1.8.1.7) is an important oxidoreductase that can protect organisms against various oxidative stresses. In this study, a new GR gene, named as HbGR2, was isolated from Hevea brasiliensis. The HbGR2 cDNA contained a 1674-bp open reading frame encoding 557 amino acids and the deduced HbGR2 protein showed high identities to the chloroplastic GRs from other plant species. HbGR2 was localized in the chloroplasts of tobacco mesophyll protoplasts. The cis-acting regulatory elements related to stress or hormone responses were predicted in the promoter region of HbGR2. The results from real-time RT-PCR analyses showed that HbGR2 was expressed throughout different tissues and developmental stages of leaves. Besides being related to tapping panel dryness (TPD), HbGR2 was regulated by several treatments including ethephon (ET), methyl jasmonate (MeJA), drought, low temperature, high salt, wounding and hydrogen peroxide (H2O2). The Escherichia coli (E. coli) cells overexpressing HbGR2 markedly increased their tolerance and survival at high concentrations of H2O2, suggesting that HbGR2 might play an important role in oxidative stress response in Hevea brasiliensis. (C) 2015 Elsevier Masson SAS. All rights reserved.