MOLECULAR STUDIES OF LYMPHOCYTES-T FROM CATTLE INFECTED WITH BOVINE LEUKEMIA-VIRUS
MOLECULAR STUDIES OF LYMPHOCYTES-T FROM CATTLE INFECTED WITH BOVINE LEUKEMIA-VIRUS
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DOI：
10.1016/0165-2427(88)90117-1
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发表时间：
1988-10-01
影响因子：
1.8
通讯作者：
AMBORSKI, GF
中科院分区：
文献类型：
--
作者：
WILLIAMS, DL;BARTA, O;AMBORSKI, GF
尽管牛白血病病毒（BLV）主要与B淋巴细胞感染有关，但我们以前曾报道过从BLV感染的无症状非白细胞血症（AL）牛中获得的T淋巴细胞在统计学上显著增加。在这份报告中，BLV前病毒的存在下，免疫亲和纯化的T淋巴细胞的DNA从AL动物进行了评估，使用高度特异性的放射性标记（32 P）BLV-DNA前病毒探针和固相DNA杂交。BLV前病毒被发现在AL动物的外周血单核细胞的DNA测试和四个纯化的T-淋巴细胞制剂从这些动物中的三个。本研究中使用的纯化T淋巴细胞制剂含有低于4%的可检测B淋巴细胞。一只动物在纯化的T淋巴细胞制备物中未检出B淋巴细胞，这些细胞的DNA也得到阳性杂交结果。淋巴细胞胚细胞生成试验，然后被用来作为一个指标的淋巴细胞的功能能力，从这些BLV感染的AL牛有丝分裂刺激的反应。当3 H-胸苷摄取（c. p.m.）用作有丝分裂诱导的胚细胞发生的测量。这表明BLV对T淋巴细胞的感染似乎不会改变淋巴细胞群体对促有丝分裂刺激的总体反应。在无促有丝分裂刺激的情况下，观察到AL动物的淋巴细胞制剂具有高水平的自发胚细胞生成。这种淋巴细胞增殖的原因尚不清楚;然而，在存在Con A、PHA和PWM的情况下，当将这些AL动物的血清添加到BLV阴性对照动物的淋巴细胞中时，发现其含有胚细胞生成增强因子。
Although bovine leukemia virus (BLV) is mainly associated with infections of B-lymphocytes we have previously reported the statistically significant increase in the T-lymphocytes obtained from BLV-infected asymptomatic aleukemic (AL) cattle. In this report the presence of BLV provirus in the DNA of immunoaffinity purified T-lymphocytes from AL animals was assessed using a highly specific radiolabeled (32P) BLV-DNA provirus probe and solid phase DNA hybridization. The BLV provirus was found in the DNA of the peripheral blood mononuclear cells of AL animals tested and three of the four purified T-lymphocyte preparations from these animals. The purified T-lymphocyte preparations used in this study contained less than 4% detectable B-lymphocytes. One animal had no detectable B-lymphocytes in the purified T-lymphocyte preparation and the DNA from these cells also gave positive hybridization results. The lymphocyte blastogenesis assay was then used as an indicator of the functional ability of lymphocytes from these BLV-infected AL cattle to respond to mitogenic stimuli. The responsiveness of lymphocytes from these animals to the mitogens concanavalin A (Con A), phytohemagglutinin (PHA), and pokeweed mitogen (PWM) was comparable to that of lymphocytes from BLV-negative animals when changes in 3H-thymidine uptake (c.p.m.) were used as measurement of mitogenic-induced blastogenesis. This indicated that infection of the T-lymphocytes by BLV does not appear to alter the overall response of the lymphocyte populations to mitogenic stimuli. High levels of spontaneous blastogenesis in the absence of mitogenic stimulation were observed for lymphocyte preparations of AL animals. The reason for this proliferation of lymphocytes is unclear; however, sera from these AL animals were found to contain a blastogenesis-augmenting factor(s) when added to lymphocytes from BLV-negative control animals in the presence of Con A, PHA and PWM.