A comparison of HIV-1 integrase inhibition by aqueous and methanol extracts of Chinese medicinal herbs
A comparison of HIV-1 integrase inhibition by aqueous and methanol extracts of Chinese medicinal herbs
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DOI：
10.1016/s0024-3205(01)00945-6
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发表时间：
2001-02-23
期刊：
影响因子：
6.1
通讯作者：
Wan, DCC
中科院分区：
文献类型：
--
作者：
Au, TK;Lam, TL;Wan, DCC
采用非放射性ELISA法检测了20种中药的水提物和甲醇提取物的抗HIV-1整合酶活性。在50 μ g/ml的草药提取物浓度下进行筛选。结果表明，大多数中药水提物和甲醇提取物对HIV-1整合酶活性有较强的抑制作用。抑制作用很可能是由于草药提取物中的单宁或多酚类物质。在大多数草药提取物中，40-80%的抗HIV-1整合酶活性可以在通过聚酰胺树脂微柱后被去除。去除多酚类化合物后，牡丹的甲醇提取物仍然对HIV-1整合酶具有强效抑制作用（EC 50 =15 μ g/ml），而夏枯草的水提取物则具有中度抑制作用（EC 50 =45 μ g/ml）。这些结果支持了草药是抗HIV化合物的丰富来源的观点。(C)2001 Elsevier Science Inc. All rights reserved.
The aqueous and methanol extracts of twenty herbs traditionally used in Chinese medicine were screened for anti-HIV-1 integrase activity in a non-radioactive ELISA-based HIV-1 integrase assay. The screening was performed at an herb extract concentration of 50 mug/ml. It was found that most of the aqueous and methanol herb extracts could elicit strong inhibition of HIV-1 integrase activity. The inhibition was most likely due to tannins or polyphenolics in the herb extracts. In most of the herb extracts, 40-80% of the anti-HIV-1 integrase activity could be removed after passing through a minicolumn of polyamide resin. After removal of polyphenolic compounds, the methanol extract of Paeonia suffruticosa still exerted potent inhibition of HIV-1 integrase (EC50=15 mug/ml) and the aqueous extract of Prunella vulgaris caused moderate inhibition (EC50=45 mug/ml). The results support the view that herbs represent a rich source of anti-HIV compounds. (C) 2001 Elsevier Science Inc. All rights reserved.