Transmitted antiretroviral drug resistance surveillance among newly HIV type 1-diagnosed women attending an antenatal clinic in Entebbe, Uganda
Transmitted antiretroviral drug resistance surveillance among newly HIV type 1-diagnosed women attending an antenatal clinic in Entebbe, Uganda
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DOI：
10.1089/aid.2007.0317
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发表时间：
2008-06-01
影响因子：
1.5
通讯作者：
Kaleebu, P.
中科院分区：
文献类型：
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作者：
Ndembi, N.;Lyagoba, F.;Kaleebu, P.
为了评估传播的HIV-1耐药性，并研究乌干达产前门诊新诊断的HIV-1菌株pol的自然多态性，我们对来自阈值监测的46株HIV-1菌株的蛋白酶和逆转录酶基因进行了测序。A1亚型占48.0%（n = 22），D亚型占39.0%（n = 18），A2亚型占2.0%（n = 1），C亚型占2.0%（n = 1），亚型间重组A1/D占9.0%（n = 4）。总体而言，在蛋白酶序列中鉴定出许多微小突变。在对我们队列的37个样本进行基因分型后，没有一个菌株与任何RTI药物或药物类别相关的主要突变。按照世卫组织准则的规定，根据艾滋病毒耐药性监测方法，估计恩德培的艾滋病毒耐药性流行率低于5%。
To evaluate transmitted HIV-1 drug resistance and study the natural polymorphism in pol of HIV-1 strains of newly diagnosed women attending an antenatal clinic in Uganda we sequenced the protease and reverse transcriptase genes for 46 HIV-1 strains from the threshold surveillance. Of the 46 sequences analyzed, 48.0% were subtype A1 (n = 22), 39.0% subtype D (n = 18), 2.0% subtype A2 (n = 1), 2.0% subtype C (n = 1), and 9.0% intersubtype recombinant A1/D (n = 4). Overall, many minor mutations were identified in the protease sequences. None of the strains had major associated mutations to any RTI drug or drug class interest after genotyping 37 samples of our cohort. The HIV drug resistance prevalence estimate in Entebbe following the HIVDR-TS methodology is less than 5% as set out by WHO guidelines.