A new NOE-mediated MT signal at around -1.6ppm for detecting ischemic stroke in rat brain.
A new NOE-mediated MT signal at around -1.6ppm for detecting ischemic stroke in rat brain.
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DOI：
10.1016/j.mri.2016.05.002
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发表时间：
2016-10
影响因子：
2.5
通讯作者：
Zu Z
中科院分区：
文献类型：
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作者：
Zhang XY;Wang F;Afzal A;Xu J;Gore JC;Gochberg DF;Zu Z
在目前的工作中，我们报告了大鼠大脑中约-1.6 ppm（NOE（-1.6））的新核奥弗豪瑟增强（NOE）介导的磁化转移（MT）信号，并研究了其在检测急性缺血性中风中的应用在啮齿动物模型中。使用连续波（CW）MT序列，在大鼠脑中可靠地检测到NOE（-1.6）。使用5池洛伦兹Z-光谱拟合方法定量大鼠脑中这种新NOE信号的振幅。在大鼠脑中使用这种拟合方法绘制酰胺、胺、−3.5 ppm NOE（NOE（−3.5））以及NOE（−1.6）的振幅。还测量了其他几个常规成像参数（R1、R2、表观扩散系数（ADC）和半固体池尺寸比（PSR））。我们的研究结果表明，NOE（-1.6），R1，R2，ADC和APT信号在卒中后0.5-1小时发生了显著变化。与其他几个成像参数相比，NOE（-1.6）显示了卒中和对侧正常组织之间最强的对比度差异，并在卒中发作后2小时内保持一致。我们的研究结果表明，这种新的NOE（-1.6）信号在大鼠脑中是一个新的潜在的对比评估急性中风在体内，并可能提供广泛的应用在其他异常组织的检测。
In the present work, we reported a new nuclear Overhauser enhancement (NOE)-mediated magnetization transfer (MT) signal at around −1.6 ppm (NOE(−1.6)) in rat brain and investigated its application in the detection of acute ischemic stroke in rodent model. Using continuous wave (CW) MT sequence, the NOE(−1.6) is reliably detected in rat brain. The amplitude of this new NOE signal in rat brain was quantified using a 5-pool Lorentzian Z-spectral fitting method. Amplitudes of amide, amine, NOE at −3.5 ppm (NOE(−3.5)), as well as NOE(−1.6) were mapped using this fitting method in rat brain. Several other conventional imaging parameters (R1, R2, apparent diffusion coefficient (ADC), and semi-solid pool size ratio (PSR)) were also measured. Our results showed that NOE(−1.6), R1, R2, ADC, and APT signals from stroke lesion have significant changes at 0.5–1 h after stroke. Compared with several other imaging parameters, NOE(−1.6) shows the strongest contrast differences between stroke and contralateral normal tissues and stays consistent over time until 2 h after onset of stroke. Our results demonstrate that this new NOE(−1.6) signal in rat brain is a new potential contrast for assessment of acute stroke in vivo and might provide broad applications in the detection of other abnormal tissues.