Evolution of polyhydroxyalkanoate (PHA) production system by "enzyme evolution": Successful case studies of directed evolution
Evolution of polyhydroxyalkanoate (PHA) production system by "enzyme evolution": Successful case studies of directed evolution
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DOI：
10.1002/mabi.200300111
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发表时间：
2004-03-15
影响因子：
4.6
通讯作者：
Doi, Y
中科院分区：
文献类型：
--
作者：
Taguchi, S;Doi, Y
通过各种代谢工程策略的发展，聚羟基烷酸酯（PHAs）生物聚酯生物合成的生物技术研究取得了广泛进展。从历史上看，利用基于外部底物操作的自然产生PHA的细菌的发酵技术（第一代）和随后的重组基因技术（第二代）实现了高效的PHA生产。最近，“酶进化”正在成为PHA生产的第三代方法。以一系列Ziegler-Natta催化剂为代表，通过“催化剂进化”开发出杰出的化学催化剂，实现了具有理想性能的大分子化学合成的突破。因此，人们可以很容易地接受这样一个概念，即与PHA合成相关的生物催化剂（酶）的分子进化将为我们创造高性能的新型PHA材料提供机会。我们小组在2001年首次报道了PHA生物合成中体外酶进化的试验。以下的文献数据，以及我们自己致力于这种新方法的实验结果，都是在短时间内积累起来的。本文着重介绍了“酶进化”在PHA生物合成中的概念和应用现状。
Biotechnological studies towards the biosynthesis of polyhydroxyalkanoates (PHAs) biopolyesters have extensively progressed through the development of various metabolic engineering strategies. Historically, efficient PHA production has been achieved using the fermentation technology of naturally occurring PHA-producing bacteria based on external substrate manipulation (1st generation), and subsequent reinforcement with recombinant gene technology (2nd generation). More recently, "enzyme evolution" is becoming the 3rd generation approach for PHA production. A break-through in the chemical synthesis of macromolecules with desirable properties was achieved by the development of prominent chemical catalysts via "catalyst evolution", as represented by a series of Ziegler-Natta catalysts. Thus, one can easily accept the concept that the molecular evolution of the biocatalysts (enzymes) relevant to PHA synthesis will provide us with a chance to create novel PHA materials with high performance. The first trial of an in vitro enzyme evolution in PHA biosynthesis was reported by our group in 2001. The following literature data, as well as our own experimental results devoted to this new approach, have been accumulated over a short time. This review article focuses specifically on the concept and current case studies of the application of "enzyme evolution" to PHA biosynthesis.