Polymorphic analysis of landraces of Japanese rice using microsatellite markers.
Polymorphic analysis of landraces of Japanese rice using microsatellite markers.
复制标题
DOI：
10.1270/jsbbr.6.125
复制
发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
Hu, J. J.
中科院分区：
文献类型：
--
作者：
Okoshi, M.;Hu JingJie, Hu JingJie;Hu, J. J.
为了确定古代日本栽培稻的特征和日本稻的起源，利用微卫星标记对73个日本稻地方品种进行了多态性分析。以4个高地棉品种和8个亚洲地方品种为标准，根据形态和生理特征将其分为籼、粳两大类。利用8个微卫星标记（RM 1、RM 20 A、RM 20 B、RM 30、RM 164、RM 167、RM 207和RM 241）进行分析。日本地方品种分为2大类群：籼型和粳型。8个地方品种属于籼型，其余为粳稻型。将粳稻分为高地组（J-A）、高地旱稻组合组（J-B）和水稻组（J-C）3个小类群。组（J-B）又分为高地（J-B1）和水稻（J-B2）两个亚组，分别对应于热带和温带粳稻。结果表明，“古早”、“泽”和“关取”高地与温带粳稻类群的水稻亲缘关系较近，可能是由水稻衍生而来的。本研究中使用的8个微卫星标记被认为是有效的指标，在日本栽培的水稻地方品种的分类和区分。
To determine the characteristics of cultivated rice in ancient Japan and the origin of Japanese rice, polymorphism analysis using microsatellite markers was carried out on 73 landraces of Japanese rice which were considered to conserve genetic diversity. Four upland Chinese varieties and eight landraces from Asia were used as standards, which had been classified into Indica and Japonica based on morphological and physiological characteristics. Eight microsatellite markers (RM1, RM20A, RM20B, RM30, RM164, RM167, RM207 and RM241) were used. Japanese landraces were classified into 2 major groups, Indica and Japonica. The 8 landraces belonged to Indica while the others belonged to Japonica. Japonica was classified into 3 minor groups; upland rice group (J-A), combined group of upland- and paddy rice (J-B) and paddy rice group (J-C). Group (J-B) was further divided into two subgroups, upland (J-B1) and paddy rice (J-B2) which correspond to tropical and temperate Japonica respectively. As the results show, "Furuwase", "Fukubouze" and "Sekitori" upland rice are closely related to paddy rice of the temperate Japonica group that may have been derived from paddy rice. The eight microsatellite markers used in this study were found to be effective indices for the classification and discrimination of the rice landraces cultivated in Japan.