Postmortem Changes in Pork Muscle Protein Phosphorylation in Relation to the RN Genotype
Postmortem Changes in Pork Muscle Protein Phosphorylation in Relation to the RN Genotype
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DOI：
10.1021/jf201936h
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发表时间：
2011-11-09
影响因子：
6.1
通讯作者：
Lindahl, Gunilla
中科院分区：
文献类型：
--
作者：
Lametsch, Rene;Larsen, Martin R.;Lindahl, Gunilla
用一维凝胶电泳法和荧光粉特异染色技术研究了死后猪肌肉蛋白磷酸化水平与RN(-)基因型的关系。发现RN(-)基因型影响几条蛋白条带的磷酸化水平，并在死后发育过程中发生变化。在受RN(-)基因影响的蛋白质条带中发现糖原磷酸化酶、磷酸果糖激酶和丙酮酸激酶。结果表明，肌肉蛋白质的磷酸化水平可以解释为包含这些酶在死后代谢中的活性状态信息的整体代谢指纹。
Postmortem changes in pork muscle protein phosphorylation in relation to the RN(-) genotype were investigated using one-dimensional gel electrophoresis and a phosphor specific staining The phosphorylation levels of several protein bands were found to be affected by the RN(-) genotype and to change during postmortem development Glycogen phosphorylase, phosphofructokinase, and pyruvate kinase were found in protein bands affected by the RN(-) genotype, and the phosphorylation profile indicates that part of the increased rate and extended pH decline of the RN(-) genotype could lie a consequence of phosphorylation of these key enzymes during the postmortem metabolism The results illustrate that the protein phosphorylation level of the muscle proteins could be interpreted as a global metabolic fingerprint containing information about the activity status of the enzymes in the postmortem metabolism