QTL Analysis for Plant Height with Molecular Markers in Maize
QTL Analysis for Plant Height with Molecular Markers in Maize
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发表时间：
2004
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影响因子：
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通讯作者：
Yan Jian;Tan G Hua;Huan Yi;Shipeng Yong;Zhenhua Yong;L. Jian
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作者：
Yan Jian;Tan G Hua;Huan Yi;Shipeng Yong;Zhenhua Yong;L. Jian
近年来，随着种植密度的增加，株高已成为重要的农艺性状之一，分子标记的快速发展为在基因组水平上定位重要的a2农艺QTL提供了有力的工具。本研究的目的是利用分子标记绘制株高 QTL 图谱并分析其在玉米中的遗传效应。以优良组合（Zong3×8721）的F2∶3群体为研究对象，采用随机完全区组设计，对武汉和襄樊两地的株高进行评价。作图群体包括266个F2∶3家系。构建了包含150个SSR和24个RFL P标记的遗传连锁图谱，总面积为2 531. 6 cm，平均间隔为14. 5 cm。采用复合区间作图法将10个影响株高的QTL定位在6条不同染色体上。 10 个 QTL 中有 7 个在两个位置被检测到。单个 QTL 对表型变异的贡献在 5. 3 % 和 17. 1 % 之间变化。检测到的影响株高的QTL均存在加性、部分显性、显性和超显性作用。通过双向方差分析检测到大量与株高相关的双基因相互作用。发现 107 和 98 两个位点组合分别在两个地点在 0. 01 概率水平下具有显着性。两个地点同时检测到其中 23 个。它们占表型变异的 4. 5 11 %。人们注意到，umc1122位点与分布在3条染色体上的其他4个不同株高位点具有双基因相互作用效应。一些株高 QTL 参与显着的双基因间相互作用，但大多数显着的相互作用发生在与定位的 QTL 不相邻的标记之间。这些结果表明，上位相互作用在决定玉米株高方面可能与单基因座效应发挥同样重要的作用。
Plant height has become one of important agronomic tra its with the increase of planting density recently and the rapid developments of molecular markers have provided powerf ul tools to local ize important a2 gronomic QTL at the genomic level . The purposes of this investigation are to map plant height QTL with molecular markers and to analyze their genetic effects in ma ize. An F2∶ 3 population from an el ite combination ( Zong3×8721) was util ized f or evaluating plant height in t wo locations , Wuhan and Xiangfan , with a ran2 domized complete block design. The mapping population included 266 F2∶ 3 family l ines. A genetic l inkage map , conta ining 150 SSR and 24 RFL P markers , was constructed , spanning a total of 2 531. 6 cm with an av2 erage interval of 14. 5 cm. Totally 10 QTL affecting plant height were mapped on six different chromosomes with the composite interval mapping. Seven of 10 QTL were detected in t wo locations. The contributions to phenotypic variations f or the single QTL varied bet ween 5. 3 and 17. 1 %. Additive , partial dominance , domi2 nance , and overdominance actions existed among all detected QTL affecting plant heights. A large number of digenic interactions f or plant height were detected by t wo2way analyses of variance. 107 and 98 t wo2locus com2 binations were f ound to be signif icant at a 0. 01 probabil ity level in t wo locations respectively. 23 of them were simultaneously detected in both locations. They accounted f or phenotypic variations of 4. 5 11 %. It was no2 ticed that a locus , umc1122 , had digenic interactive effects with other f our different loci f or plant height , which distributed on three chromosomes. A fe w of plant height QTL was involved in signif icant digenic inter2 actions , but most signif icant interactions occurred bet ween markers that are not adjacent to mapped QTL. These results demonstrated that epistatic interactions might play an equal importance role as the single2locus effects in determining plant height of ma ize.