Molecular cloning and characterization of a novel stem-specific gene from Camptotheca acuminata.
Molecular cloning and characterization of a novel stem-specific gene from Camptotheca acuminata.
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DOI：
10.5483/bmbrep.2006.39.1.068
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发表时间：
2006-01
期刊：
影响因子：
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通讯作者：
Y. Pi;Z. Liao;Y. Chai;Hainian Zeng;Peng Wang;Y. Gong;Y. Pang;Xiaofen Sun;K. Tang
中科院分区：
文献类型：
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作者：
Y. Pi;Z. Liao;Y. Chai;Hainian Zeng;Peng Wang;Y. Gong;Y. Pang;Xiaofen Sun;K. Tang
在高等植物中，p450参与许多重要的次生代谢产物的生物合成。本文首次采用RACE-PCR技术，从中国本土药用植物喜树（Camptotheca acuminata，简称CaSS）中分离到一段新的细胞色素P450 cDNA，该基因以茎特异性方式表达。cDNA全长1735 bp，包含一个1530 bp的开放阅读框（ORF），编码509个氨基酸的多肽。生物信息学分析显示，CASS含有一个血红素结合结构域PFGXGRRXCX，与其他植物细胞色素P450单加氧酶和羟化酶具有同源性。Southern blotting分析表明，喜树基因组中只存在一个CaSS拷贝。Northern blotting分析显示，CaSS在茎中表达量高，在根、叶和花中表达量低，具有组织特异性。我们的研究表明，CaSS可能参与了苯丙素途径。
In higher plants, P450s participate in the biosynthesis of many important secondary metabolites. Here we reported for the first time the isolation of a new cytochrome P450 cDNA that expressed in a stem-specific manner from Camptotheca acuminata (designated as CaSS), a native medicinal plant species in China, using RACE-PCR. The full-length cDNA of CaSS was 1735 bp long containing a 1530 bp open reading frame (ORF) encoding a polypeptide of 509 amino acids. Bioinformatic analysis revealed that CASS contained a heme-binding domain PFGXGRRXCX and showed homology to other plant cytochrome P450 monooxygenases and hydroxylases. Southern blotting analysis revealed that there was only one copy of the CaSS present in the genome of Camptotheca acuminata. Northern blotting analysis revealed that CaSS expressed, in a tissuespecific manner, highly in stem and lowly in root, leaf and flower. Our study suggests that CaSS is likely to be involved in the phenylpropanoid pathway.