Thermoresponsive Epitope Surface-Imprinted Nanoparticles for Specific Capture and Release of Target Protein from Human Plasma
Thermoresponsive Epitope Surface-Imprinted Nanoparticles for Specific Capture and Release of Target Protein from Human Plasma
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用于从人血浆中特异性捕获和释放目标蛋白的热响应表位表面印迹纳米颗粒
DOI：
10.1021/acsami.5b11415
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发表时间：
2016-03-09
影响因子：
9.5
通讯作者：
Zhang, Yukui
中科院分区：
文献类型：
--
作者：
Li, Senwu;Yang, Kaiguang;Zhang, Yukui
在各种人工抗体中，表位印迹聚合物受到越来越多的关注。利用N-异丙基丙烯酰胺调节环境刺激下的“吸附与释放”行为，制备热响应性表位印迹位点。所制备的印迹材料可吸附46.6 mg/g的目标蛋白，印迹因子为4.0。模板利用率最高可达8.21%。更重要的是，在真实样本中，该材料可以在45℃可控地从人体血浆中捕获目标蛋白，并在4℃下将其释放，这表明了此类材料在生物分子识别领域的按需应用潜力。
Among various artificial antibodies, epitope imprinted polymer has been paid increasingly attention. To modulate the "adsorption and release" behavior by environment stimuli, N-isopropylacrylamide, was adopted to fabricate the thermoresponsive epitope imprinted sites. The prepared imprinted materials could adsorb 46.6 mg/g of target protein with the imprinting factor of 4.0. The template utilization efficiency could reach as high as 8.21%. More importantly, in the real sample, the materials could controllably capture the target protein from the human plasma at 45 degrees C and release it at 4 degrees C, which demonstrated the "on-demand" application potentials of such materials in the biomolecule recognition field.