The accuracy of ribosomal RNA comparative structure models
The accuracy of ribosomal RNA comparative structure models
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DOI：
10.1016/s0959-440x(02)00339-1
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发表时间：
2002-06-01
影响因子：
6.8
通讯作者：
Cannone, JJ
中科院分区：
文献类型：
--
作者：
Gutell, RR;Lee, JC;Cannone, JJ
在20世纪70年代末确定了首批完整的16S和23S rRNA序列之后不久，便开始了16S和23S rRNA二级结构模型的确定工作。通过对数千条rRNA序列进行分析并使用比较方法，确定了所有16S rRNAs和所有23S rRNAs共有的结构。二十多年后，针对近期确定的30S和50S核糖体亚基的高分辨率晶体结构，对16S和23S rRNA比较结构模型进行了评估。几乎所有基于预测的协同变异的碱基对，包括规则碱基对和螺旋，以及不规则碱基对和三级相互作用，都存在于30S和50S晶体结构中。
The determination of the 16S and 23S rRNA secondary structure models was initiated shortly after the first complete 16S and 23S rRNA sequences were determined in the late 1970s. The structures that are common to all 16S rRNAs and all 23S rRNAs were determined using comparative methods from the analysis of thousands of rRNA sequences. Twenty-plus years later, the 16S and 23S rRNA comparative structure models have been evaluated against the recently determined high-resolution crystal structures of the 30S and 50S ribosomal subunits. Nearly all of the predicted covariation-based base pairs, including the regular base pairs and helices, and the irregular base pairs and tertiary interactions, were present in the 30S and 50S crystal structures.