Substrate protein dependence of GroEL-GroES interaction cycle revealed by high-speed atomic force microscopy imaging
Substrate protein dependence of GroEL-GroES interaction cycle revealed by high-speed atomic force microscopy imaging
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DOI：
10.1098/rstb.2017.0180
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发表时间：
2018-06-19
影响因子：
6.3
通讯作者：
Ando, Toshio
中科院分区：
文献类型：
--
作者：
Noshiro, Daisuke;Ando, Toshio
双环形十四聚体GroEL复合物与辅伴侣蛋白GroES合作协助适当的蛋白质折叠。动态GroEL-GroES相互作用反映了环内和环间的变构通信和伴侣蛋白反应。因此，揭示这种动态的相互作用对于理解GroEL的变构通讯和运作机制至关重要。然而，这种相互作用如何在伴侣蛋白循环中进行一直存在争议。在这里，我们直接使用高速原子力显微镜在有和没有可折叠底物蛋白的条件下对动态GroEL-GroES相互作用进行成像。然后，在这些条件下获得的成像结果和我们以前的结果在展开基板的存在下进行了比较。分子电影显示，整个反应途径是高度复杂的，但基本上是相同的，无论底物的条件。一个显著的（但中等的）差异是中间物种的群体分布：对称GroEL：GroES（2）和不对称GroEL：GroES（1）复合物，以及GroES-未结合的GroEL。这种差异主要归因于在可折叠衬底存在下GroEL：GroES 1复合物的较长寿命。此外，环间通信，这是两个环的交替作用的基础，发生在两个不同的（GroES协会和解离）的步骤中的主要反应途径，无论基板condition.This文章是讨论会议的一部分问题“Allostery和分子机器”。
A double-ring-shaped tetradecameric GroEL complex assists proper protein folding in cooperation with the cochaperonin GroES. The dynamic GroEL-GroES interaction reflects the allosteric intra-and inter-ring communications and the chaperonin reaction. Therefore, revealing this dynamic interaction is essential to understanding the allosteric communications and the operation mechanism of GroEL. Nevertheless, how this interaction proceeds in the chaperonin cycle has long been controversial. Here, we directly image the dynamic GroEL-GroES interaction under conditions with and without foldable substrate protein using high-speed atomic force microscopy. Then, the imaging results obtained under these conditions and our previous results in the presence of unfoldable substrate are compared. The molecular movies reveal that the entire reaction pathway is highly complicated but basically identical irrespective of the substrate condition. A prominent (but moderate) difference is in the population distribution of intermediate species: symmetric GroEL : GroES(2) and asymmetric GroEL : GroES(1) complexes, and GroES-unbound GroEL. This difference is mainly attributed to the longer lifetime of GroEL : GroES1 complexes in the presence of foldable substrate. Moreover, the inter-ring communication, which is the basis for the alternating action of the two rings, occurs at two distinct (GroES association and dissociation) steps in the main reaction pathway, irrespective of the substrate condition.This article is part of a discussion meeting issue 'Allostery and molecular machines'.