Direct Link between Toluene Degradation in Contaminated-Site Microcosms and a Polaromonas Strain
Direct Link between Toluene Degradation in Contaminated-Site Microcosms and a Polaromonas Strain
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DOI：
10.1128/aem.01364-09
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发表时间：
2009-12
影响因子：
4.4
通讯作者：
Weimin Sun;Shuguang Xie;Chunling Luo;A. Cupples
中科院分区：
文献类型：
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作者：
Weimin Sun;Shuguang Xie;Chunling Luo;A. Cupples
摘要采用稳定同位素探针法对土壤微生态系统中的好氧甲苯降解菌进行了鉴定。几种方法(末端限制性片段长度多态性、16S rRNA基因测序和定量聚合酶链式反应)提供了证据，表明致病微生物是极光单胞菌属的成员，可以在甲苯上生长。这种微生物也能转化苯，但不能转化间二甲苯或顺式二氯乙烯。
ABSTRACT Stable isotope probing (SIP) was used to identify the aerobic toluene-degrading microorganism in soil microcosms. Several approaches (terminal restriction fragment length polymorphism, 16S rRNA gene sequencing, and quantitative PCR) provided evidence that the microorganism responsible was a member of the genus Polaromonas and could grow on toluene. This microorganism also transformed benzene, but not m-xylene or cis-dichloroethene.