Regulation of the anaphase-promoting complex by the dual specificity phosphatase human Cdc14a
Regulation of the anaphase-promoting complex by the dual specificity phosphatase human Cdc14a
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DOI：
10.1074/jbc.m108126200
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发表时间：
2001-12-21
影响因子：
4.8
通讯作者：
Yu, HT
中科院分区：
文献类型：
--
作者：
Bembenek, J;Yu, HT
两种形式的后期促进复合物（APC）介导的关键细胞周期调节剂的降解。APC（Cdc 20）通过泛素化securin促进姐妹染色单体分离，而APC（Cdh 1）泛素化有丝分裂细胞周期蛋白，允许退出有丝分裂。在这里，我们表明，磷酸化的人Cdh 1（hCdh 1）的细胞周期蛋白B-Cdc 2改变了hCdh 1的构象，并阻止它激活APC。酵母Cdc 14的人类同源物，人Cdc 14 a（hCdc 14 a），使hCdh 1去磷酸化并激活APC（Cdh 1）。相反，hCdc 14 a不影响APC（Cdc 20）的活性。hCdc 14 a是hCdh 1的主要磷酸酶，定位于HeLa细胞的中心体。因此，hCdc 14 a可能促进APC（Cdh 1）的活化，并退出有丝分裂。马里细胞。
Two forms of the anaphase-promoting complex (APC) mediate the degradation of critical cell cycle regulators. APC(Cdc20) promotes sister-chromatid separation by ubiquitinating securin, whereas APC(Cdh1) ubiquitinates mitotic cyclins, allowing the exit from mitosis. Here we show that phosphorylation of human Cdh1 (hCdh1) by cyclin B-Cdc2 alters the conformation of hCdh1 and prevents it from activating APC. A human homologue of yeast Cdc14, human Cdc14a (hCdc14a), dephosphorylates hCdh1 and activates APC(Cdh1). In contrast, hCdc14a does not affect the activity of APC(Cdc20). hCdc14a is a major phosphatase for hCdh1 and localizes to centrosomes in HeLa cells. Therefore, hCdc14a may promote the activation of APC(Cdh1) and exit from mitosis in mam. malian cells.