Sulfolipid density dictates the extent of carbon nanodot interaction with chloroplast membranes
Sulfolipid density dictates the extent of carbon nanodot interaction with chloroplast membranes
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DOI：
10.1039/d2en00158f
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发表时间：
2022-06-17
影响因子：
7.3
通讯作者：
Pedersen，Joel A.
中科院分区：
文献类型：
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作者：
Kim，Kyoungtea;Jeon，Su-Ji;Pedersen，Joel A.
纳米材料输送到植物叶绿体的机制已经被探索，以提高植物的抗逆性，促进光合作用，促进基因工程，并制造自修复生物材料、燃料和生物药物。然而，纳米材料与叶绿体膜之间的分子相互作用还不是很清楚。在这项研究中，我们研究了一组重要的叶绿体膜脂，包括磺基喹诺酮二甘油与不同官能团电荷的碳纳米点的相互作用。为了实现这一目标，我们构建了一种新型的叶绿体膜模型，并利用具有耗散监测的石英晶体微天平研究了碳纳米点功能基团电荷、模型叶绿体膜组成和离子强度对碳纳米点-叶绿体膜相互作用的影响。我们进一步利用共聚焦激光扫描显微镜研究了碳纳米点与从拟南芥中分离的天然叶绿体的相互作用。我们的结果表明，碳纳米点-叶绿体膜的相互作用主要是由静电决定的。尽管是叶绿体膜中最不丰富的脂类，我们发现模型膜中磺基喹诺酮二酰甘油的相对丰度是控制膜与正电荷碳纳米点相互作用的亲和力和能力的成分。碳纳米点在模型叶绿体膜上的附着速率随离子强度的变化而变化，其方式与碳纳米点上的双电层压缩一致。我们的发现在分子水平上阐明了纳米材料与植物生物表面之间的化学相互作用，并可能有助于建立可持续纳米材料与植物细胞器膜的结构-性质-相互作用关系。
Mechanisms of nanomaterial delivery to plant chloroplasts have been explored to improve plant stress tolerance, promote photosynthesis, facilitate genetic engineering, and manufacture self-repairing biomaterials, fuels, and biopharmaceuticals. However, the molecular interactions of nanomaterials with chloroplast membranes are not well understood. In this study, we examine the interactions of an important set of chloroplast membrane lipids including sulfoquinovosyl diacylglycerols with carbon nanodots varying in functional group charge. To accomplish this objective, we constructed a novel model chloroplast membrane and interrogated the influence of carbon nanodot functional group charge, model chloroplast membrane composition, and ionic strength on the carbon nanodot-chloroplast membrane interactions using a quartz crystal microbalance with dissipation monitoring. We further examined the interaction of carbon nanodots with native chloroplasts isolated from Arabidopsis thaliana using confocal laser-scanning microscopy. Our results indicate that carbon nanodot-chloroplast membrane interactions are dictated primarily by electrostatics. Despite being the least abundant lipids in chloroplast membranes, we find that the relative abundance of sulfoquinovosyl diacylglycerol in model membranes is the component that governs both the affinity for and capacity of the membrane to interact with positively charged carbon nanodots. Rates of carbon nanodot attachment to model chloroplast membranes varied with ionic strength in a manner consistent with electrical double layer compression on carbon nanodots. Our findings elucidate chemical interactions between nanomaterials and plant biosurfaces at the molecular level and potentially contribute to establishing structure–property-interaction relationships of sustainable nanomaterials with plant organelle membranes.