INTERACTION OF ASSEMBLED PROGENY POX VIRUSES WITH THE CELLULAR CYTOSKELETON
INTERACTION OF ASSEMBLED PROGENY POX VIRUSES WITH THE CELLULAR CYTOSKELETON
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DOI：
10.1016/0042-6822(79)90533-6
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发表时间：
1979-01-01
期刊：
影响因子：
3.7
通讯作者：
JUNGWIRTH, C
中科院分区：
文献类型：
--
作者：
HILLER, G;WEBER, K;JUNGWIRTH, C
通过免疫荧光显微镜和电镜研究了感染牛痘病毒或牛痘病毒的鸡胚纤维母细胞。两种技术都表明成熟的子代病毒与细胞骨架的含有肌动蛋白的微丝密切相关。在感染后期，细胞显示出特殊的微绒毛型结构，其中包含微丝核心，其尖端有病毒颗粒。在通常发现与细胞微丝中的肌动蛋白相关的蛋白质（原肌球蛋白、肌球蛋白和α-肌动蛋白）中，只有α-肌动蛋白可以被肯定地鉴定为与这些痘病毒诱导的微绒毛内的肌动蛋白丝阵列相关。这些特殊的微绒毛可以很容易地与正常的细胞微绒毛区分开来，因为它们的直径（容纳痘病毒颗粒所需的宽度）大了近 3 倍。病毒组装的抑制阻止了特化微绒毛的诱导，因此表明成熟病毒或仅在这种条件下存在的因子诱导其形成。除了在微绒毛中发现的病毒颗粒外，还观察到许多其他成熟的病毒颗粒与靠近质膜的微丝结构相连。
Chick embryo friboblasts infected with vaccinia WR or cowpox virus were studied by immunofluorescence microscopy and EM. Both techniques indicate an intimate association of mature progeny virus with the actin-containing microfilaments of the cellular cytoskeleton. Late in infection cells display specialized microvilli-type structures which contain a core of microfilaments with a virus particle at their tip. Of the proteins usually found associated with actin in cellular microfilaments (tropomyosin, myosin and .alpha.-actinin) only .alpha.-actinin could be positively identified as associated with the array of actin filaments within these poxvirus-induced microvilli. These specialized microvilli can easily be distinguished from normal cellular microvilli because they are nearly 3-fold larger in diameter, the width necessary to accommodate a pox virion. Inhibition of virus assembly prevents the induction of the specialized microvilli, thus indicating that mature viruses, or a factor present only under such conditions, induce their formation. In addition to virus particles found in microvilli, numerous other mature virions are observed connected to the microfilamentous structure which lie close to the plasma membrane.