Mechanism for the separation of large molecules based on radial migration in capillary electrophoresis
Mechanism for the separation of large molecules based on radial migration in capillary electrophoresis
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DOI：
10.1021/ac034430u
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发表时间：
2003-08-01
影响因子：
7.4
通讯作者：
Yeung, ES
中科院分区：
文献类型：
--
作者：
Zheng, JJ;Yeung, ES
在毛细管电泳法中，我们提出了一种新的基于径向迁移的大分子分离机制。径向迁移的方向取决于外加HDF相对于电场的方向。径向运移速度是尺寸相关的，这可以归因于剪切流作用下不同程度的变形。在含有；的样品塞上演示了分析分离。DNA(48 502碱基)和phiX174 RF DNA(5386碱基)，基线分离。或者，该分离模式可以连续进行，因此适用于制备性分离。这种分离技术不需要凝胶/聚合物或复杂的仪器，是毛细管凝胶电泳和场流分离的补充。虽然这里使用了大的DNA分子来演示分离机制，但这些方案也可以应用于基于大小、形状或变形能力的蛋白质、细胞或颗粒的分离。
We demonstrate a novel separation mechanism for large molecules based on their radial migration in capillary electrophoresis with applied hydrodynamic flow (HDF). The direction of radial migration depends on the direction of the applied HDF relative to the electric field. The radial migration velocities are size-dependent, which could be attributed to the different degree of deformation under shear flow. Analytical separation was demonstrated on a sample plug containing;. DNA (48 502 bp) and phiX174 RF DNA (5386 bp) with baseline separation. Alternatively, this separation mode can be performed continuously and is thus applicable to preparative separations. Without the need for gel/polymer or complex instrumentation, this separation technique is complementary to capillary gel electrophoresis and field-flow fractionation. Although large DNA molecules were used to demonstrate the separation mechanism here, these protocols could also be applied to the separation of proteins, cells, or particles based on size, shape, or deformability.