Selected Ion Flow Tube Mass Spectrometry Analysis of Volatile Metabolites in Urine Headspace for the Profiling of Gastro-Esophageal Cancer
Selected Ion Flow Tube Mass Spectrometry Analysis of Volatile Metabolites in Urine Headspace for the Profiling of Gastro-Esophageal Cancer
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DOI：
10.1021/ac4000656
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发表时间：
2013-03-19
影响因子：
7.4
通讯作者：
Hanna, George B.
中科院分区：
文献类型：
--
作者：
Huang, Juzheng;Kumar, Sacheen;Hanna, George B.
尿液被认为是一种理想的临床研究生物流体，因为它是非侵入性的，而且相对大容量的尿液很容易获得。本研究应用选择离子流动管质谱(SIFT-MS)对三组胃食道癌患者、上消化道非癌疾病患者和健康人群的尿液顶空蒸气中的挥发性有机化合物(VOCs)进行了定量研究。本研究共调查了11种挥发性有机化合物。使用Kruskal-Wallis检验发现，七种VOCs-乙醛、丙酮、醋酸、己酸、硫化氢、甲醇和苯酚的浓度在癌症组、阳性对照组和健康组之间有显著差异。与健康对照组相比，癌症队列中乙醛、丙酮、醋酸、己酸、硫化氢和甲醇浓度升高，而苯酚浓度下降。乙醇、丙醇、甲基苯酚和乙基苯酚的浓度在癌症组和对照组之间没有显著差异。受试者操作特征(ROC)分析应用于6种VOCs(乙醛、丙酮、醋酸、己酸、硫化氢和甲醇)的组合，以区分癌症患者和非癌症对照。ROC曲线下的积分面积为0.904。这一结果表明，VOC谱可能适合于识别胃食道癌的高危人群。因此，应该进行进一步的研究，以评估VOC图谱作为一种新的胃食道癌筛查试验的潜力。
Urine is considered an ideal biofluid for clinical investigation because it is obtained noninvasively and relatively large volumes are easily acquired. In this study, selected ion flow tube mass spectrometry (SIFT-MS) has been applied for the quantification of volatile organic compounds (VOCs) in the headspace vapor of urine samples, which were retrieved from three groups of patients with gastro-esophageal cancer, noncancer diseases of the upper gastro-intestinal tract, and a healthy cohort. Eleven VOCs have been investigated in this study. The concentrations of seven VOCs-acetaldehyde, acetone, acetic acid, hexanoic acid, hydrogen sulfide, methanol, and phenol-were found to be significantly different between cancer, positive control, and healthy groups using the Kruskal-Wallis test. The concentrations of acetaldehyde, acetone, acetic acid, hexanoic acid, hydrogen sulfide, and methanol were increased in the cancer cohort compared with healthy controls while the concentration of phenol decreased. The differences in the concentrations of ethanol, propanol, methyl phenol, and ethyl phenol were not significant between cancer and control groups. Receiver operating characteristics (ROC) analysis was applied for a combination of six VOCs (acetaldehyde, acetone, acetic acid, hexanoic acid, hydrogen sulfide, and methanol) to discriminate cancer patients from noncancer controls. The integrated area under ROC curve is 0.904. This result indicates that VOC profiling may be suitable in identifying those at high risk of gastro-esophageal cancer. Therefore, further investigations should be undertaken to assess the potential for VOC profiling as a new screening test in gastro-esophageal cancer.