Mismatch tolerance by DNA polymerase Pol4 in the course of nonhomologous end joining in Saccharomyces cerevisiae
Mismatch tolerance by DNA polymerase Pol4 in the course of nonhomologous end joining in Saccharomyces cerevisiae
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DOI：
10.1534/genetics.105.053512
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发表时间：
2006-04-01
期刊：
影响因子：
3.3
通讯作者：
Marcand, S
中科院分区：
文献类型：
--
作者：
Pardo, B;Ma, E;Marcand, S
在酵母中，非同源末端连接途径（NHEJ）动员DNA聚合酶Pol 4修复DNA双链断裂时，需要连接前的间隙填充。使用由端粒蛋白Rap 1的丢失和转化DNA上的双链断裂修复引起的端粒-端粒融合作为完全非粘性末端之间的NHEJ的测定，我们表明Pol 4能够延伸最后一个碱基错配的3 '末端，即，错配对或未配对的，与模板链。
In yeast, the nonhomologous end joining pathway (NHEJ) mobilizes the DNA polymerase Pol4 to repair DNA double-strand breaks when gap filling is required prior to ligation. Using telomere-telomere fusions caused by loss of the telomeric protein Rap1 and double-strand break repair on transformed DNA as assays for NHEJ between fully uncohesive ends, we show that Pol4 is able to extend a 3'-end whose last bases are mismatched, i.e., mispaired or unpaired, to the template strand.