Glucose transporter plasticity during memory processing
Glucose transporter plasticity during memory processing
复制标题
DOI：
10.1016/j.neuroscience.2004.09.011
复制
发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Messier, C
中科院分区：
文献类型：
--
作者：
Choeiri, C;Staines, W;Messier, C
各种类型的学习，包括操作性条件反射，诱导细胞激活增加，同时增加局部脑葡萄糖利用（LCGU）。这种增加是由脑血流量增加或脑毛细血管密度和直径的变化介导的。由于葡萄糖转运蛋白最终负责葡萄糖的摄取，我们研究了它们在细胞激活时的可塑性表达。体外和体内研究表明，脑葡萄糖转运蛋白1 （GLUT1）的表达与LCGU的变化一致。本研究首次探讨了记忆加工对葡萄糖转运蛋白表达的影响。在CD1小鼠的大脑中测量了操作性条件反射任务训练产生的GLUT表达的变化。采用半定量免疫组织化学、Western blot和实时RT-PCR方法，在训练后220 min和24 h立即定量测定大脑GLUT1和GLUT3的表达。相对于假训练和初始对照，操作性条件反射训练诱导海马CA1锥体细胞和感觉运动皮层中GLUT1免疫反应水平立即增加。在较长的学习后延迟中，感觉运动皮层和壳核的GLUT1免疫反应性下降。与蛋白质水平的变化平行，海马GLUT1 mRNA水平也在学习后立即升高。学习对海马GLUT3蛋白和mRNA表达没有影响。葡萄糖转运蛋白表达的变化表明细胞活化和葡萄糖代谢之间存在联系。本研究中观察到的学习诱导的GLUT1蛋白和mRNA水平的局部增加证实了之前的发现，即GLUT1的表达具有可塑性，并响应细胞代谢需求的变化。(c) 2005年。Elsevier Ltd.出版。版权所有。
Various types of learning, including operant conditioning, induce an increase in cellular activation concomitant with an increase in local cerebral glucose utilization (LCGU). This increase is mediated by increased cerebral blood flow or changes in brain capillary density and diameter. Because glucose transporters are ultimately responsible for glucose uptake, we examined their plastic expression in response to cellular activation. In vitro and in vivo studies have demonstrated that cerebral glucose transporter 1 (GLUT1) expression consistently parallels changes in LCGU. The present study is the first to investigate the effect of memory processing on glucose transporters expression. Changes in GLUT expression produced by training in an operant conditioning task were measured in the brain of CD1 mice. Using semi-quantitative immunohistochemistry, Western blot and real time RT-PCR the cerebral GLUT1 and GLUT3 expression was quantified immediately, 220 min and 24 h following training. Relative to sham-trained and naive controls, operant conditioning training induced an immediate increase in GLUT1 immunoreactivity level in the hippocampus CA1 pyramidal cells as well as in the sensorimotor cortex. At longer post-learning delays, GLUT1 immunoreactivity decreased in the sensorimotor cortex and putamen. Parallel to the changes in protein levels, hippocampus GLUT1 mRNA level also increased immediately following learning. No effect of learning was found on hippocampal GLUT3 protein or mRNA expression. Measures of changes in glucose transporters expression present a link between cellular activation and glucose metabolism. The learning-induced localized increases in GLUT1 protein as well as mRNA levels observed in the present study confirm the previous findings that GLUT1 expression is plastic and respond to changes in cellular metabolic demands. (C) 2005 IBRO. Published by Elsevier Ltd. All rights reserved.