Phenol oxidases from Rhodnius prolixus: Temporal and tissue expression pattern and regulation by ecdysone
Phenol oxidases from Rhodnius prolixus: Temporal and tissue expression pattern and regulation by ecdysone
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DOI：
10.1016/j.jinsphys.2010.03.027
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发表时间：
2010-09-01
影响因子：
2.2
通讯作者：
Azambuja, P.
中科院分区：
文献类型：
--
作者：
Genta, F. A.;Souza, R. S.;Azambuja, P.
红花5龄若虫在前中肠、脂肪体和血淋巴中有显著的PO酶活性。PO活性最大的组织是前中肠，比活性较高的组织是脂肪体和血淋巴。在这项工作中，研究了不同组织中PO酶活性的时间模式。在脂肪体中，PO峰值出现在血餐后的第7、12和16天。血淋巴PO减少至第7天，第14天恢复。在前中肠组织中，PO在第9天和透析前达到峰值；在前中肠内容物中也观察到类似的模式。其中一些活动依赖于蜕皮激素的释放，因为喂养含有印楝素的血粉会抑制它们，而蜕皮激素治疗会抵消这种作用。这些结果表明，在5龄昆虫的发育过程中，存在基础PO表达和激活的自然调节周期，这可能与自然感染的发生有关。印楝素和蜕皮素在各器官中活性的时间模式和作用的差异表明，至少在长叶姜中，不同的组织表达不同的PO基因。(C) 2010 Elsevier Ltd.版权所有。
Rhodnius prolixus 5th instar nymphs have significant PO enzymatic activity in the anterior midgut, fat body and hemolymph. The tissue with the major amount of PO activity is the anterior midgut while those with higher specific activities are the fat body and hemolymph. In this work the temporal pattern of PO enzymatic activity in different tissues was investigated. In fat body, PO peaks occur at 7, 12 and 16 days after a blood meal. In hemolymph, PO diminishes until day 7, and then recovers by day 14. In the anterior midgut tissue, PO peaks on day 9 and just before ecdysis; a similar pattern was observed in the anterior midgut contents. Some of these activities are dependent on the release of ecdysone, as feeding blood meal containing azadirachtin suppresses them and ecdysone treatment counteracts this effect. These results suggest that during the development of the 5th instar, the insect has natural regulating cycles of basal PO expression and activation, which could be related to the occurrence of natural infections. The differences in temporal patterns of activity and the effects of azadirachtin and ecdysone in each organ suggest that, at least in R. prolixus, different tissues are expressing different PO genes. (C) 2010 Elsevier Ltd. All rights reserved.