Sperm sexing in sheep and cattle: The exception and the rule
Sperm sexing in sheep and cattle: The exception and the rule
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DOI：
10.1016/j.theriogenology.2008.09.014
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发表时间：
2009-01-01
期刊：
影响因子：
2.8
通讯作者：
Maxwell, W. M. C.
中科院分区：
文献类型：
--
作者：
de Graaf, S. P.;Beilby, K. H.;Maxwell, W. M. C.
流式细胞分选技术在性别预选方面取得了长足的进步。这项技术已经使许多物种能够生产出性别前的后代，并在世界各地的牛身上取得了商业上的成功。然而。由于性别分选过程中固有的压力，性别分选精子在雌性生殖道内的受精寿命受到了广泛的功能损害，其结果是运动能力和生存能力降低，功能状态改变。与未分选对照相比，这些特征在体内表现为较低的生育力。然而。技术的改进和对其生物学影响的更深入了解促进了最近在绵羊方面的发展。表明性别分选能够在体外和体内功能方面选择功能优越的种群。这些结果在其他物种的最新进展背景下进行了回顾，并讨论了用性别分类的公羊精子人工授精成功的原因。(C) 2008爱思唯尔公司版权所有。
Flow cytometric sorting for the preselection of sex has progressed considerably in the 20 years since its inception. This technique has allowed the production of pre-sexed offspring in a multitude of species and become a commercial success in cattle around the world. However. due to the stress inherent to the sex-sorting process, sex-sorted spermatozoa are widely recognized as functionally compromised in terms of their fertilizing lifespan within the female reproductive tract as a result of reduced motility and viability and changed functional state. These characteristics, when compared to non-sorted controls, are manifest in vivo as lower fertility. However. improvements to the technology and a greater understanding of its biological impact have facilitated recent developments in sheep. showing sex-sorting is capable of selecting a functionally superior population in terms of both in vitro and in vivo function. These results are reviewed in the context of recent developments in other species and the reasons for Success after artificial insemination with sex-sorted ram spermatozoa are discussed. (C) 2008 Elsevier Inc. All rights reserved.