Novel linear quadrupole ion trap/FT mass spectrometer: Performance characterization and use in the comparative analysis of histone H3 post-translational modifications
Novel linear quadrupole ion trap/FT mass spectrometer: Performance characterization and use in the comparative analysis of histone H3 post-translational modifications
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DOI：
10.1021/pr0499794
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发表时间：
2004-05-01
影响因子：
4.4
通讯作者：
Hunt, DF
中科院分区：
文献类型：
--
作者：
Syka, JEP;Marto, JA;Hunt, DF
我们描述了一个原型高性能混合质谱仪的设计和性能。该仪器由线性四极离子阱（QLT）耦合到傅立叶变换离子回旋共振质量分析器（FTMS）。这种配置提供了快速和自动化的MS和MS/MS分析，类似于在标准3-D Paul阱上发现的“数据依赖扫描”，但具有显著改进的内部扫描动态范围、质量测量精度、质量分辨率和检测限。描述了在zeptomole水平上的肽的序列分析。该仪器最近发布的商业版本在LC/MS模式下运行（1秒/扫描），质量分辨率为100 000，并配备自动增益控制，可在没有内标的情况下提供1-2 ppm的质量测量准确度。方法描述，使用该仪器来比较组蛋白H3分离的异步生长的细胞和细胞在有丝分裂停滞的翻译后修饰。
We describe the design and performance of a prototype high performance hybrid mass spectrometer. This instrument consists of a linear quadrupole ion trap (QLT) coupled to a Fourier transform ion cyclotron resonance mass analyzer (FTMS). This configuration provides rapid and automated MS and MS/MS analyses, similar to the "data dependent scanning" found on standard 3-D Paul traps, but with substantially improved internal scan dynamic range, mass measurement accuracy, mass resolution, and detection limits. Sequence analysis of peptides at the zeptomole level is described. The recently released, commercial version of this instrument operates in the LC/MS mode (1 s/scan) with a mass resolution of 100 000 and is equipped with automatic gain control to provide mass measurement accuracy of 1-2 ppm without internal standard. Methodology is described that uses this instrument to compare the post-translational modifications present on histone H3 isolated from asynchronously growing cells and cells arrested in mitosis.