Wax and cutin mutants of Arabidopsis: Quantitative characterization of the cuticular transport barrier in relation to chemical composition.
Wax and cutin mutants of Arabidopsis: Quantitative characterization of the cuticular transport barrier in relation to chemical composition.
复制标题
DOI：
10.1016/j.bbalip.2016.03.002
复制
发表时间：
2016-09
期刊：
影响因子：
--
通讯作者：
C. Sadler;Bettina Schroll;Viktoria Zeisler;F. Waßmann;R. Franke;L. Schreiber
中科院分区：
文献类型：
--
作者：
C. Sadler;Bettina Schroll;Viktoria Zeisler;F. Waßmann;R. Franke;L. Schreiber
以14 C标记的氟环唑为示踪剂，定量测定了拟南芥（Arabidopsis thaliana）叶片表皮透性，并比较了蜡质和角质突变体（wax 2、cut 1、cer 5、att 1、bdg、shn 3和shn 1）与相应野生型（Col-0和Ws）的差异。突变体的特征在于蜡和/或角质量的减少或增加。与野生型相比，突变体中拟南芥的渗透性[m s-1]增加或不受影响。因此，蜡和角质生物合成的遗传变化在一些调查的拟南芥突变体明显损害协调角质和蜡沉积在外叶表皮细胞壁。因此，铜的阻隔性能显著降低。然而，通过基因修饰增加角质和蜡的量，并不自动导致改善的表皮屏障性能。作为一种替代方法的放射性运输测定，叶绿素荧光的变化进行了监测后，叶面施用嗪，除草剂抑制叶绿体中的电子传递。由于这两个，光合作用抑制的一半时间，以及完全抑制的时间，实际上与14 C-氟环唑渗透，不同的速率之间的光合产量的下降突变体和野生型必须是一个功能的叶片吸收的除草剂通过角质层。因此，监测叶绿素荧光的变化，而不是进行放射性运输测定，代表了一种易于处理和快速的替代方法，评估不同基因型的表皮屏障特性。本文是由肯特D. Chapman和Ivo Feussner。
Using14C-labeled epoxiconazole as a tracer, cuticular permeability of Arabidopsis thaliana leaves was quantitatively measured in order to compare different wax and cutin mutants (wax2,cut1,cer5,att1,bdg,shn3andshn1) to the corresponding wild types (Col-0 and Ws). Mutants were characterized by decreases or increases in wax and/or cutin amounts. Permeances [m s− 1] of Arabidopsis cuticles either increased in the mutants compared to wild type or were not affected. Thus, genetic changes in wax and cutin biosynthesis in some of the investigated Arabidopsis mutants obviously impaired the coordinated cutin and wax deposition at the outer leaf epidermal cell wall. As a consequence, barrier properties of cuticles were significantly decreased. However, increasing cutin and wax amounts by genetic modifications, did not automatically lead to improved cuticular barrier properties. As an alternative approach to the radioactive transport assay, changes in chlorophyll fluorescence were monitored after foliar application of metribuzine, an herbicide inhibiting electron transport in chloroplasts. Since both, half-times of photosynthesis inhibition as well as times of complete inhibition, in fact correlated with14C-epoxiconazole permeances, different rates of decline of photosynthetic yield between mutants and wild type must be a function of foliar uptake of the herbicide across the cuticle. Thus, monitoring changes in chlorophyll fluorescence, instead of conducting radioactive transport assays, represents an easy-to-handle and fast alternative evaluating cuticular barrier properties of different genotypes. This article is part of a Special Issue entitled: Plant Lipid Biology edited by Kent D. Chapman and Ivo Feussner.