Western-Type Helicobacter pylori CagA are the Most Frequent Type in Mongolian Patients-猫眼课题宝

DOI：

10.3390/cancers11050725

发表时间：

2019-05

期刊：

Cancers

影响因子：

5.2

通讯作者：

中科院分区：

医学2区

文献类型：

--

作者：

Tegshee Tserentogtokh;B. Gantuya;Phawinee Subsomwong;Khasag Oyuntsetseg;Dashdorj Bolor;Yansan Erdene-Ochir;Dashdorj Azzaya;D. Davaadorj;T. Uchida;T. Matsuhisa;Y. Yamaoka

具有东亚型CagA的幽门螺杆菌感染与癌症发生相关蒙古的胃癌死亡率最高。因此，我们评估了蒙古族人群中的CagA状态。通过内镜检查和组织学检查确定高危人群和胃癌患者。H. pylori菌株分离自蒙古的不同地点。CagA亚型（东亚型或西方型，基于Glu-Pro-Ile-Tyr-Ala（EPIYA）片段的测序）和vacA基因型（s和m区域）分别使用基于PCR的测序和PCR确定。总共检查了368名患者（341名胃炎，10名消化性溃疡和17名胃癌）。cagA阴性62株（16.8%），阳性306株（83.1%），其中西方型293株，东亚型12株，混合型1株。所有cagA阴性菌株均分离自胃炎患者。在胃炎组中，78.6%（268/341）为西方型CagA，2.9%（10/341）为东亚型，18.2%（61/341）为cagA阴性。然而，所有H.胃癌患者pylori中的CagA蛋白为Western型。组织学分析表明东亚型CagA是最强的毒株，其次是西方型和cagA阴性毒株。这一发现与目前的共识一致。CagA阳性菌株为最强毒力型。然而，不同的CagA类型可以解释胃癌的高发病率，这一事实可能不适用于蒙古。

Helicobacter pylori infection possessing East-Asian-type CagA is associated with carcinogenesis. Mongolia has the highest mortality rate from gastric cancer. Therefore, we evaluated the CagA status in the Mongolian population. High risk and gastric cancer patients were determined using endoscopy and histological examination. H. pylori strains were isolated from different locations in Mongolia. The CagA subtypes (East-Asian-type or Western-type, based on sequencing of Glu-Pro-Ile-Tyr-Ala (EPIYA) segments) and vacA genotypes (s and m regions) were determined using PCR-based sequencing and PCR, respectively. In total, 368 patients were examined (341 gastritis, 10 peptic ulcer, and 17 gastric cancer). Sixty-two (16.8%) strains were cagA-negative and 306 (83.1%) were cagA-positive (293 Western-type, 12 East-Asian-type, and one hybrid type). All cagA-negative strains were isolated from gastritis patients. In the gastritis group, 78.6% (268/341) had Western-type CagA, 2.9% (10/341) had East-Asian-type, and 18.2% (61/341) were cagA-negative. However, all H. pylori from gastric cancer patients possessed Western-type CagA. Histological analyses showed that East-Asian-type CagA was the most virulent strains, followed by Western-type and cagA-negative strains. This finding agreed with the current consensus. CagA-positive strains were the most virulent type. However, the fact that different CagA types can explain the high incidence of gastric cancer might be inapplicable in Mongolia.