Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii
Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii
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DOI：
10.1104/pp.104.041400
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发表时间：
2004-07-01
期刊：
影响因子：
7.4
通讯作者：
Fukuzawa, H
中科院分区：
文献类型：
--
作者：
Miura, K;Yamano, T;Fukuzawa, H
光合适应CO2限制胁迫与控制的遗传和生理反应，通过信号转导途径，其次是综合监测环境变化。虽然之前已经分离出了几个CO2响应基因，但尚未将全基因组分析应用于分离可能作为光合真核生物中碳浓缩机制（CCM）的一部分发挥作用的CO2响应基因。利用含有10，368个表达序列标签的cDNA膜阵列比较了在富CO2条件下生长的细胞和在CO2限制条件下生长的细胞的表达谱，检测到莱茵衣藻中51个低CO2诱导基因和32个低CO抑制基因的mRNA水平变化超过2.5倍。事实上，几乎所有的低CO2诱导基因的诱导受损的CCM 1突变体表明，CCM 1是一个主调节CCM通过假定的低CO2信号转导途径。在低CO2诱导的基因中，发现了两个新的基因，LciA和LciB，它们可能参与无机碳的运输。低CO2诱导和/或CCM 1调节基因的可能功能进行了讨论，在CCM。
Photosynthetic acclimation to CO2-limiting stress is associated with control of genetic and physiological responses through a signal transduction pathway, followed by integrated monitoring of the environmental changes. Although several CO2-responsive genes have been previously isolated, genome-wide analysis has not been applied to the isolation of CO2-responsive genes that may function as part of a carbon-concentrating mechanism (CCM) in photosynthetic eukaryotes. By comparing expression profiles of cells grown under CO2-rich conditions with those of cells grown under CO2-limiting conditions using a cDNA membrane array containing 10,368 expressed sequence tags, 51 low-CO2 inducible genes and 32 genes repressed by low CO, whose mRNA levels were changed more than 2.5-fold in Chlamydomonas reinhardtii Dangeard were detected. The fact that the induction of almost all low-CO2 inducible genes was impaired in the ccm1 mutant suggests that CCM1 is a master regulator of CCM through putative low-CO2 signal transduction pathways. Among low-CO2 inducible genes, two novel genes, LciA and LciB, were identified, which may be involved in inorganic carbon transport. Possible functions of low-CO2 inducible and/or CCM1-regulated genes are discussed in relation to the CCM.