Comparative full genome analysis revealed E1: A226V shift in 2007 Indian Chikungunya virus isolates
Comparative full genome analysis revealed E1: A226V shift in 2007 Indian Chikungunya virus isolates
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DOI：
10.1016/j.virusres.2008.02.004
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发表时间：
2008-07-01
期刊：
影响因子：
5
通讯作者：
Rao, P. V. Lakshmana
中科院分区：
文献类型：
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作者：
Santhosh, S. R.;Dash, P. K.;Rao, P. V. Lakshmana
基孔肯雅病毒（CHIKV）在印度时隔32年后以前所未有的爆炸性流行形式死灰复燃，这是一个重大的公共卫生问题。2007年，印度喀拉拉邦再次爆发疫情，影响了2.5万多例病例，其中许多人死亡。为了了解这种高毒力的分子基础及其在大规模流行中的意义，对喀拉拉邦2007年的流行样本进行了详细的系统血清学、病毒学和分子调查。2007年基孔肯雅病毒分离株与2006年基孔肯雅病毒分离株的全基因组序列比较分析发现，2007年基孔肯雅病毒分离株的结构和非结构基因有3个独特的替换[2个在El区（V14A和A226V）， 1个在Nsp1区（M184T）]。我们的发现进一步证实了El基因A226V的转移与进化成功的关联，这可能是由于蚊子媒介对流行病进展的适应，正如在留尼汪岛观察到的那样。在本研究分析的2007年4个分离株中发现了在2006年所有印度分离株中不存在的A226V转移。随着2005年至2007年疫情的发展，2007年分离株的这些独特分子特征表明它们具有很高的进化和流行潜力，从而表明此次疫情的规模和毒性可能更高。(c) 2008 Elsevier B.V.版权所有
The resurgence of Chikungunya virus (CHIKV) in the form of unprecedented explosive epidemic after a gap of 32 years in India is a point of major public health concern. In 2007 again there was outbreak in Kerala, India, affecting more than 25,000 cases with many reported mortalities. To understand the molecular basis of this high virulence and its implication in large-scale epidemic, a detailed systematic serological, virological and molecular investigation was undertaken with the epidemic samples of Kerala-2007. The comparative analysis of full genome sequence of Chikungunya virus isolate of 2007 with 2006 revealed three unique substitutions in structural and non-structural genes of 2007 isolate [two in El region (V14A and A226V) and one in Nsp1 (M184T)]. Our finding further substantiates the association of A226V shift in El gene with evolutionary success possibly due to adaptation in the mosquito vector with progression of epidemic, as observed in Reunion Island. This A226V shift which was absent in all 2006 Indian isolates, is found to be present in the four 2007 isolates, analysed in this study. These unique molecular features of the 2007 isolates with the progression of the epidemic from 2005 to 2007 demonstrate their high evolutionary and epidemic potential and thereby suggesting possible implication in higher magnitude and virulence of this outbreak. (c) 2008 Elsevier B.V. All rights reserved.