Maternal Ribosomes Are Sufficient for Tissue Diversification during Embryonic Development in C-elegans
Maternal Ribosomes Are Sufficient for Tissue Diversification during Embryonic Development in C-elegans
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DOI：
10.1016/j.devcel.2019.01.019
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发表时间：
2019-03-25
影响因子：
11.8
通讯作者：
Fire, Andrew
中科院分区：
文献类型：
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作者：
Cenik, Elif Sarinay;Meng, Xuefeng;Fire, Andrew
秀丽隐杆线虫提供了一个顺从的系统来探索新合成的核糖体是否需要通过发育来进步。尽管在胚胎发育过程中形成了复杂的组织模式，但我们发现，缺乏五种不同核糖体蛋白(RPS)中任何一种的纯合子都可以产生功能完全正常的第一阶段幼虫，在完全缺失多拷贝核糖体RNA基因座后，可以看到类似的发育能力。这些动物依靠母体而不是合子核糖体成分的贡献，能够完成胚胎发生。在缺乏新的核糖体成分的情况下，所产生的动物在从第一个幼虫阶段进展之前就被阻止了，并且在两次神经元结构的胚胎后可塑性测试中失败。对幼虫的镶嵌分析表明，在一种全球调控机制中，部分细胞中的核糖体不足会引发整个生物体的生长停滞。
Caenorhabditis elegans provides an amenable system to explore whether newly composed ribosomes are required to progress through development. Despite the complex pattern of tissues that are formed during embryonic development, we found that null homozygotes lacking any of the five different ribosomal proteins (RPs) can produce fully functional first-stage larvae, with similar developmental competence seen upon complete deletion of the multi-copy ribosomal RNA locus. These animals, relying on maternal but not zygotic contribution of ribosomal components, are capable of completing embryogenesis. In the absence of new ribosomal components, the resulting animals are arrested before progression from the first larval stage and fail in two assays for postembryonic plasticity of neuronal structure. Mosaic analyses of larvae that are a mixture of ribosome-competent and non-competent cells suggest a global regulatory mechanism in which ribosomal insufficiency in a subset of cells triggers organism-wide growth arrest.