Prediction of Cd and Pb toxicity to Vibrio fischeri using biotic ligand-based models in soil
Prediction of Cd and Pb toxicity to Vibrio fischeri using biotic ligand-based models in soil
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DOI：
10.1016/j.jhazmat.2011.11.085
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发表时间：
2012-02-15
影响因子：
13.6
通讯作者：
Nam, Kyoungphile
中科院分区：
文献类型：
--
作者：
An, Jinsung;Jeong, Seulki;Nam, Kyoungphile
采用费氏弧菌毒性试验，建立了Cd、Pb污染土壤的生物配体毒性预测模型。首先，阳离子的竞争效应（即，Ca、Mg、K）的影响。为此，基于生物配体的模型参数包括阳离子和金属离子与结合位点的条件结合常数（即，生物配体）和金属离子占据的结合位点的分数进行了测定。水相毒性试验数据表明，模型预测的Cd和Pb的EC 50值与实验测定的EC 50值之间的差异在2倍以内，表明所建立的模型参数是可靠的。其次，使用土壤溶液来预测镉和铅的土壤毒性进行了实验验证与新鲜加标和现场老化的土壤。结果表明，在两种土壤中的线性关系，这意味着土壤溶液的毒性可以代表土壤的毒性。最后，通过比较预测的毒性效应（即，在土壤溶液中给定的阳离子和金属活性下的生物发光抑制%）和通过固相毒性试验测定的实验获得的毒性效应。我们的数据表明，使用开发的基于生物配体的模型，以土壤溶液的化学分析数据为输入数据，可以预测镉或铅污染土壤的毒性。(C)2011 Elsevier B.V.保留所有权利。
Biotic ligand-based models to predict site-specific toxicity of Cd and Pb contaminated soil were developed by using a Vibrio fischeri toxicity test. Firstly, competition effect by cations (i.e., Ca, Mg, K) commonly found in soil solution was incorporated into the models. For this purpose, biotic ligand-based model parameters including conditional binding constants of cations and metal ions to binding sites (i.e., biotic ligands) and the fractions of binding sites occupied by the metal ions were determined. Data from aqueous phase toxicity test showed that the difference between model-predicted EC50 values of Cd and Pb and experimentally determined EC50 values ranged within a factor of two, suggesting that the developed model parameters were reliable. Secondly, the use of soil solution to predict soil toxicity of Cd and Pb was experimentally verified with freshly spiked and field-aged soils. The results showed linear relationships in both soils, meaning that toxicity of soil solution can be representative of toxicity of soil. Finally, applicability of the developed models in Cd- or Pb-spiked soils was investigated by comparing predicted toxic effects (i.e., % bioluminescence inhibition at given cations and metal activities in soil solution) and experimentally obtained toxic effects determined by Microtox (R) solid phase toxicity test. Our data demonstrate that toxicity of Cd- or Pb-contaminated soil can be predicted by using the developed biotic ligand-based model with the chemical analysis data of soil solution as input data. (C) 2011 Elsevier B.V. All rights reserved.