Infection of non-encapsulated species of Trichinella ameliorates experimental autoimmune encephalomyelitis involving suppression of Th17 and Th1 response
Infection of non-encapsulated species of Trichinella ameliorates experimental autoimmune encephalomyelitis involving suppression of Th17 and Th1 response
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DOI：
10.1007/s00436-010-1985-9
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发表时间：
2010-07
影响因子：
2
通讯作者：
Zhiliang Wu;I. Nagano;K. Asano;Y. Takahashi
中科院分区：
文献类型：
--
作者：
Zhiliang Wu;I. Nagano;K. Asano;Y. Takahashi
流行病学和实验研究表明，蠕虫感染可以改善自身免疫性疾病。本研究探讨了旋毛虫感染对实验性自身免疫性脑脊髓炎（Experimental Autoimmune encephalomyelitis，EAE）的改善作用以及在EAE发病机制中起关键作用的Th 17和Th 1细胞因子的表达动力学。结果表明，T.假螺旋体能明显改善EAE的临床症状，并能明显延迟髓鞘少突胶质细胞糖蛋白（MOG）免疫诱导的EAE的发病。感染引起的炎症浸润和脱髓鞘在感染的EAE小鼠的中枢神经系统比未感染的EAE小鼠少得多。炎性细胞因子IL-17、IL-6、IL-1β、IFN-γ和TNF-α的表达也表明了浸润的减少，这些细胞因子在未感染EAE小鼠的脊髓中较高，而在感染EAE小鼠中接近正常或较低。MOG再刺激EAE小鼠后，可抑制MOG诱导的IL-17和IFN-γ的产生，抑制IL-6、IL-1β、TGF-β的表达。另一方面，在感染EAE的小鼠的脾细胞中观察到强烈诱导的Th 2应答。本研究表明T.假螺旋体感染可通过减少中枢神经系统的炎症浸润来抑制EAE，这可能与感染抑制Th 17和Th 1应答有关。
Epidemiological and experimental studies have indicated that helminth infections can ameliorate autoimmune diseases. The present study investigated the amelioration effect of theTrichinella pseudospiralisinfection on experimental autoimmune encephalomyelitis (EAE), a T-cell-mediated autoimmune disease of central nervous system (CNS), and expression kinetics of Th17 and Th1 cytokine which play a crucial role in the pathogenesis of EAE. The results indicated that the infection of helminthT. pseudospiralisobviously ameliorated clinical severity and greatly delayed the onset of EAE induced by myelin oligodendrocyte glycoprotein (MOG) immunization. Infection caused much lesser inflammatory infiltration and demyilination in the CNS of infected EAE mice than uninfected EAE mice. The reduced infiltration was also suggested by the expressions of the inflammation cytokines, IL-17, IL-6, IL-1β, IFN-γ, and TNF-α, which were high in the spinal cords of the uninfected EAE mice, but was nearly normal or low in the infected EAE mice. The increased production of MOG-induced IL-17 and IFN-γ and the expression of IL-6, IL-1β, TGF-β in splenocytes after restimulation with MOG was inhibited in the infected EAE mice. On the other hand, the greatly induced Th2 response was observed in the splenocytes of the infected EAE mice. The present study showed thatT. pseudospiralisinfection can suppresses EAE by reducing the inflammatory infiltration in CNS, likely associated with the suppression of Th17 and Th1 responses by the infection.