Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposite
Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposite
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基于生物相容性羧甲基壳聚糖-金纳米粒子纳米复合物的辣根过氧化物酶的直接电化学
DOI：
10.1016/j.bios.2006.02.010
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发表时间：
2006-12-15
影响因子：
12.6
通讯作者：
Sheng, Jian
中科院分区：
文献类型：
--
作者：
Xu, Qin;Mao, Chun;Sheng, Jian
采用一种新颖的原位法制备了羧甲基壳聚糖(CMCS)与金纳米颗粒复合的纳米复合材料。用透射电子显微镜和傅里叶变换红外光谱仪对其进行了表征。该纳米复合材料即使在中性溶液中也是亲水性的，稳定性好，继承了AuNPs和CMCs的特性，使其具有生物相容性，可用于酶的固定化。将HRP作为模型酶固定在含有纳米复合材料的硅胶-凝胶基质上，构建了一种新型的过氧化氢生物传感器。实现了HRP的直接电子转移，并进行了研究。该生物传感器具有快速的安培响应(5 S)，在5.0×10~(-6)~1.4×10~(-3)M浓度范围内具有良好的线性响应，检测下限为4.01×10~(-7)M，表观米氏常数(K-M(App))为5.7×10~(-4)M。(C)2006爱思唯尔B.V.保留所有权利。
The nanocomposite composed of carboxymethyl chitosan (CMCS) and gold nanoparticles was successfully prepared by a novel and in situ process. It was characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectrophotometer (FTIR). The nanocomposite was hydrophilic even in neutral solutions, stable and inherited the properties of the AuNPs and CMCS, which make it biocompatible for enzymes immobilization. HRP, as a model enzyme, was immobilized on the silica sol-gel matrix containing the nanocomposite to construct a novel H2O2 biosensor. The direct electron transfer of HRP was achieved and investigated. The biosensor exhibited a fast amperometric response (5 s), a good linear response over a wide range of concentrations from 5.0 x 10(-6) to 1.4 x 10(-3) M, and a low detection limit of 4.01 x 10(-7) M. The apparent Michaelis-Menten constant (K-M(app)) for the biosensor was 5.7 x 10(-4) M. Good stability and sensitivity were assessed for the biosensor. (c) 2006 Elsevier B.V. All rights reserved.