Improved therapeutic drug monitoring of tacrolimus (FK506) by tandem mass spectrometry.
Improved therapeutic drug monitoring of tacrolimus (FK506) by tandem mass spectrometry.
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通过串联质谱法改进了他克莫司 (FK506) 的治疗药物监测。
DOI：
10.1093/clinchem/43.11.2189
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发表时间：
1997
影响因子：
9.3
通讯作者：
S. Pond
中科院分区：
文献类型：
--
作者：
P. Taylor;N. Hogan;S. Lynch;A. G. Johnson;S. Pond
免疫抑制药物他克莫司(FK506)的效力是环孢素A的50-100倍，已被证明在防止实体器官移植中的排斥反应方面非常有效。然而，由于他克莫司的治疗范围狭窄、药动学可变和潜在的药物相互作用，他克莫司的持续治疗药物监测(TDM)是必不可少的。最近，我们发表了一份报告，详细介绍了一种特异、灵敏的血液中他克莫司浓度定量方法的开发(3)。与现有的免疫分析方法相比，该方法具有更高的特异性、更低的检测限和更快的周转时间。这些特性使这一方法学成为他克莫司TDM的理想方法。
自从我们的第一份报告以来，已经实施了几项进一步改进TDM检测的修改。使用100×2 mm C8柱(而不是30×2 mm C4柱)，与…相结合
The immunosuppressant drug tacrolimus (FK506), which exhibits 50–100 times the potency of cyclosporin A, is proving to be highly effective in preventing rejection in solid-organ transplantation (1). However, because of a narrow therapeutic range, variable pharmacokinetics, and potential drug interactions, continual therapeutic drug monitoring (TDM) of tacrolimus is essential (2). Recently, we published a report that detailed the development of a specific, sensitive method for quantification of tacrolimus concentrations in blood (3). This methodology, which utilizes HPLC in combination with tandem mass spectrometry (LC-MS2), was found to have greater specificity, lower detection limits, and a more rapid turnaround time than existing immunoassays. These attributes make this methodology ideal for TDM of tacrolimus.
Since our initial report, several modifications that have further improved the assay for TDM have been implemented. The use of a 100 × 2 mm C8 column (rather than a 30 × 2 mm C4 column), combined with …