PSEUDOMONAS-AERUGINOSA ISOLATES FROM PATIENTS WITH CYSTIC-FIBROSIS - A CLASS OF SERUM-SENSITIVE, NONTYPABLE STRAINS DEFICIENT IN LIPOPOLYSACCHARIDE-O SIDE-CHAINS
PSEUDOMONAS-AERUGINOSA ISOLATES FROM PATIENTS WITH CYSTIC-FIBROSIS - A CLASS OF SERUM-SENSITIVE, NONTYPABLE STRAINS DEFICIENT IN LIPOPOLYSACCHARIDE-O SIDE-CHAINS
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DOI：
10.1128/iai.42.1.170-177.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
PIER, GB
中科院分区：
文献类型：
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作者：
HANCOCK, REW;MUTHARIA, LM;PIER, GB
对囊性纤维化患者分离的26株铜绿假单胞菌按Fisher免疫分型方案进行分型。只有6株菌株与单一分型血清凝集，15株与~gt；1血清凝集，5株不与任何血清凝集。血凝抑制试验和免疫扩散试验均不能分型，说明这些多凝菌株不表达多种血清型抗原，而是被抗非血清型决定簇抗体凝集。4株可分型分离株对混合正常人血清具有抵抗力；所研究的12株多凝和非凝集分离株对正常血清非常敏感。对16株细菌的外膜进行分离和鉴定。提出了外膜蛋白模式的高度保守性。采用一种新的技术对脂多糖进行了纯化，该技术可以高产率地分离粗大、光滑的脂多糖。在检测的4株可分型、抗血清的菌株中，3株含有O抗原的光滑内毒素与实验室野生型铜绿假单胞菌PAO1株H103相当。在12株多凝或非凝集的血清敏感菌株中，12株含有很少或没有光滑的O抗原内毒素；另外2株含有比野生型H103更少的光滑内毒素。菌株H103的5个独立的、粗略的、LPSO抗原缺陷的突变体是不能分型的，对血清敏感。显然，这里描述的脂多糖缺陷代表了许多与囊性纤维化相关的铜绿假单胞菌菌株的重要新特性。
P. aeruginosa strains (26) from patients with cystic fibrosis were typed by the Fisher immunotyping scheme. Only 6 strains were agglutinated by a single typing serum; 15 strains were agglutinated with > 1 serum and 5 were not agglutinated by any serum. Neither the polyagglutinable nor the nonagglutinable strains were typable by hemagglutination inhibition or immunodiffusion, suggesting that these polyagglutinable strains did not express multiple serotype antigen, but were instead being agglutinated by antibody to nonserotype determinants. Four typable isolates were resistant to pooled normal human serum; the 12 polyagglutinable and nonagglutinable isolates studied were very sensitive to normal serum. The outer membranes of 16 strains were isolated and characterized. Strong conservation of outer membrane protein patterns was suggested. Lipopolysaccharides (LPS) were purified by a new technique which allowed isolation of rough and smooth LPS in high yields. Of 4 typable, serum-resistant strains examined, 3 had amounts of smooth, O-antigen-containing LPS equivalent to a laboratory wild-type, P. aeruginosa PAO1 strain H103. Of 12 polyagglutinable or nonagglutinable, serum-sensitive strains, 12 had very little or no smooth, O-antigen-containing LPS; the other 2 contained less smooth LPS than the wild-type strain H103. Five independent, rough, LPS O-antigen-deficient mutants of strain H103 were nontypable and serum-sensitive. Evidently, the LPS defects described here represent a significant new property of many P. aeruginosa strains associated with cystic fibrosis.